2026-05-22T00:57:24Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/142552024-09-27T20:17:43Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609
Repisalud
author
Garcia-Rios, Estefani
author
Gata-de-Benito, Julia
author
Lopez-Siles, Mireia
author
McConnell, Michael J
author
Perez-Romero, Pilar
funder
Ministerio de Ciencia, Innovación y Universidades (España)
funder
Instituto de Salud Carlos III
2022-05-05T08:42:18Z
2022-05-05T08:42:18Z
2021-09-29
Int J Mol Sci. 2021 Sep 29;22(19):10558.
10.3390/ijms221910558
1422-0067
International Journal of Molecular Sciences
34638896
http://hdl.handle.net/20.500.12105/14255
Human cytomegalovirus (HCMV) continues to be a major cause of morbidity in transplant patients and newborns. However, the functions of many of the more than 282 genes encoded in the HCMV genome remain unknown. The development of bacterial artificial chromosome (BAC) technology contributes to the genetic manipulation of several organisms including HCMV. The maintenance of the HCMV BAC in E. coli cells permits the rapid generation of recombinant viral genomes that can be used to produce viral progeny in cell cultures for the study of gene function. We optimized the Lambda-Red Recombination system to construct HCMV gene deletion mutants rapidly in the complete set of tested genes. This method constitutes a useful tool that allows for the quick generation of a high number of gene deletion mutants, allowing for the analysis of the whole genome to improve our understanding of HCMV gene function. This may also facilitate the development of novel vaccines and therapeutics.
eng
BAC
Lambda-RED system
Cytomegalovirus
Gene-deletion mutants
Recombination
Optimization of a Lambda-RED Recombination Method for Rapid Gene Deletion in Human Cytomegalovirus
journal article
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OptimizationOfALambda_2021.pdf
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OptimizationOfALambda Supplementary Materials_2021.pdf
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OptimizationOfALambda_2021.pdf.txt
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OptimizationOfALambda Supplementary Materials_2021.pdf.txt