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00925njm 22002777a 4500 dc Garcia-Rios, Estefani author Gata-de-Benito, Julia author Lopez-Siles, Mireia author McConnell, Michael J author Perez-Romero, Pilar author 2021-09-29 Human cytomegalovirus (HCMV) continues to be a major cause of morbidity in transplant patients and newborns. However, the functions of many of the more than 282 genes encoded in the HCMV genome remain unknown. The development of bacterial artificial chromosome (BAC) technology contributes to the genetic manipulation of several organisms including HCMV. The maintenance of the HCMV BAC in E. coli cells permits the rapid generation of recombinant viral genomes that can be used to produce viral progeny in cell cultures for the study of gene function. We optimized the Lambda-Red Recombination system to construct HCMV gene deletion mutants rapidly in the complete set of tested genes. This method constitutes a useful tool that allows for the quick generation of a high number of gene deletion mutants, allowing for the analysis of the whole genome to improve our understanding of HCMV gene function. This may also facilitate the development of novel vaccines and therapeutics. Int J Mol Sci. 2021 Sep 29;22(19):10558. 10.3390/ijms221910558 1422-0067 International Journal of Molecular Sciences 34638896 http://hdl.handle.net/20.500.12105/14255 BAC Lambda-RED system Cytomegalovirus Gene-deletion mutants Recombination Optimization of a Lambda-RED Recombination Method for Rapid Gene Deletion in Human Cytomegalovirus