2026-06-14T03:52:26Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/141602024-11-28T17:47:18Zcom_20.500.12105_2052com_20.500.12105_2051com_20.500.12105_15322col_20.500.12105_19609col_20.500.12105_16961
Repisalud
author
Fehér, Csaba
author
Pastor-Ibáñez, Roque
author
Leal, Lorna
author
Plana, Montserrat
author
Arnedo, Mireia
author
van den Ham, Henk-Jan
author
Andeweg, Arno C
author
Gruters, Rob A
author
Díez-Fuertes, Francisco
author
Alcamí, José
author
Aloy, Patrick
author
García, Felipe
funder
Government of Catalonia (España)
funder
Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF)
funder
Ministerio de Economía (España)
funder
Instituto de Salud Carlos III
funder
Unión Europea. Comisión Europea
funder
Red de Investigación Cooperativa en Investigación en Sida (España)
2022-04-22T15:47:49Z
2022-04-22T15:47:49Z
2021-07-19
Vaccines (Basel). 2021;9(7):799.
10.3390/vaccines9070799
2076-393X
Vaccines
34358215
http://hdl.handle.net/20.500.12105/14160
Systems vaccinology has seldomly been used in therapeutic HIV-1 vaccine research. Our aim was to identify early gene 'signatures' that predicted virus load control after analytical therapy interruption (ATI) in participants of a dendritic cell-based HIV-1 vaccine trial (DCV2). mRNA and miRNA were extracted from frozen post-vaccination PBMC samples; gene expression was determined by microarray method. In gene set enrichment analysis, responders showed an up-regulation of 14 gene sets (TNF-alpha/NFkB pathway, inflammatory response, the complement system, Il6 and Il2 JAK-STAT signaling, among others) and a down-regulation of 7 gene sets (such as E2F targets or interferon alpha response). The expression of genes regulated by three (miR-223-3p, miR-1183 and miR-8063) of the 9 differentially expressed miRNAs was significantly down-regulated in responders. The deregulation of certain gene sets related to inflammatory processes seems fundamental for viral control, and certain miRNAs may be important in fine-tuning these processes.
eng
Dendritic cell-based therapeutic HIV-1 vaccine
Differential gene expression
Gene set enrichment analysis
miRNA
mMRA
Association of Transcriptomic Signatures of Inflammatory Response with Viral Control after Dendritic Cell-Based Therapeutic Vaccination in HIV-1 Infected Individuals
journal article
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AssociationOfTranscriptomicSignatures_2021.pdf
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AssociationOfTranscriptomicSignaturesSupplementary_2021.pdf
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AssociationOfTranscriptomicSignatures_2021.pdf.txt
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AssociationOfTranscriptomicSignaturesSupplementary_2021.pdf.txt