2026-06-14T03:56:04Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/141602024-11-28T17:47:18Zcom_20.500.12105_2052com_20.500.12105_2051com_20.500.12105_15322col_20.500.12105_19609col_20.500.12105_16961

00925njm 22002777a 4500 dc Fehér, Csaba author Pastor-Ibáñez, Roque author Leal, Lorna author Plana, Montserrat author Arnedo, Mireia author van den Ham, Henk-Jan author Andeweg, Arno C author Gruters, Rob A author Díez-Fuertes, Francisco author Alcamí, José author Aloy, Patrick author García, Felipe author 2021-07-19 Systems vaccinology has seldomly been used in therapeutic HIV-1 vaccine research. Our aim was to identify early gene 'signatures' that predicted virus load control after analytical therapy interruption (ATI) in participants of a dendritic cell-based HIV-1 vaccine trial (DCV2). mRNA and miRNA were extracted from frozen post-vaccination PBMC samples; gene expression was determined by microarray method. In gene set enrichment analysis, responders showed an up-regulation of 14 gene sets (TNF-alpha/NFkB pathway, inflammatory response, the complement system, Il6 and Il2 JAK-STAT signaling, among others) and a down-regulation of 7 gene sets (such as E2F targets or interferon alpha response). The expression of genes regulated by three (miR-223-3p, miR-1183 and miR-8063) of the 9 differentially expressed miRNAs was significantly down-regulated in responders. The deregulation of certain gene sets related to inflammatory processes seems fundamental for viral control, and certain miRNAs may be important in fine-tuning these processes. Vaccines (Basel). 2021;9(7):799. 10.3390/vaccines9070799 2076-393X Vaccines 34358215 http://hdl.handle.net/20.500.12105/14160 Dendritic cell-based therapeutic HIV-1 vaccine Differential gene expression Gene set enrichment analysis miRNA mMRA Association of Transcriptomic Signatures of Inflammatory Response with Viral Control after Dendritic Cell-Based Therapeutic Vaccination in HIV-1 Infected Individuals