2026-07-21T15:05:39Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/141312025-06-13T09:32:03Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19617
Repisalud
author
Arévalo, Beatriz
author
Blázquez-García, Marina
author
Valverde, Alejandro
author
Serafín, Verónica
author
Montero-Calle, Ana Maria
author
Solis-Fernandez, Guillermo
author
Barderas Manchado, Rodrigo
author
Campuzano, Susana
author
Yáñez-Sedeño, Paloma
author
Pingarrón, José M
funder
Ministerio de Ciencia y Universidades (España)
funder
Ministerio de Economía e Innovación (España)
funder
Comunidad de Madrid (España)
funder
Instituto de Salud Carlos III
funder
Ministerio de Educación, Cultura y Deporte (España)
funder
Research Foundation - Flanders
funder
Conferencia de Rectores de las Universidades Españolas
2022-04-20T09:56:35Z
2022-04-20T09:56:35Z
2022-03-14
Mikrochim Acta. 2022;189(4):143.
10.1007/s00604-022-05250-4
1436-5073
Mikrochimica Acta
35286499
http://hdl.handle.net/20.500.12105/14131
A dual immunosensor is reported for the simultaneous determination of two important immunity-related cytokines: BAFF (B cell activation factor) and APRIL (a proliferation-induced signal). Sandwich-type immunoassays with specific antibodies (cAbs) and a strategy for signal amplification based on labelling the detection antibodies (dAbs) with binary MoS2/MWCNTs nanostructures and using horseradish peroxidase (HRP) were implemented. Amperometric detection was carried out at screen-printed dual carbon electrodes (SPdCEs) through the hydroquinone HQ/H2O2 system. The developed dual immunosensor provided limit of detection (LOD) of 0.08 and 0.06 ng mL-1 for BAFF and APRIL, respectively, and proved to be useful for the determination of both cytokines in cancer cell lysates and serum samples from patients diagnosed with autoimmune diseases and cancer. The obtained results agreed with those found using ELISA methodologies.
eng
APRIL
Amperometry
BAFF
CRC
Dual immunoplatform
MoS2/MWCNTs
SLE
Serum
Binary MoS2 nanostructures as nanocarriers for amplification in multiplexed electrochemical immunosensing: simultaneous determination of B cell activation factor and proliferation-induced signal immunity-related cytokines.
research article
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BinaryMoS2Nanostructures_2022.pdf
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https://repisalud.isciii.es/bitstreams/7f5f23a8-4b66-43b0-8019-5e8e0e24627a/download
File
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text/plain
BinaryMoS2Nanostructures_2022.pdf.txt