2026-07-21T15:05:39Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/141312025-06-13T09:32:03Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19617

00925njm 22002777a 4500 dc Arévalo, Beatriz author Blázquez-García, Marina author Valverde, Alejandro author Serafín, Verónica author Montero-Calle, Ana Maria author Solis-Fernandez, Guillermo author Barderas Manchado, Rodrigo author Campuzano, Susana author Yáñez-Sedeño, Paloma author Pingarrón, José M author 2022-03-14 A dual immunosensor is reported for the simultaneous determination of two important immunity-related cytokines: BAFF (B cell activation factor) and APRIL (a proliferation-induced signal). Sandwich-type immunoassays with specific antibodies (cAbs) and a strategy for signal amplification based on labelling the detection antibodies (dAbs) with binary MoS2/MWCNTs nanostructures and using horseradish peroxidase (HRP) were implemented. Amperometric detection was carried out at screen-printed dual carbon electrodes (SPdCEs) through the hydroquinone HQ/H2O2 system. The developed dual immunosensor provided limit of detection (LOD) of 0.08 and 0.06 ng mL-1 for BAFF and APRIL, respectively, and proved to be useful for the determination of both cytokines in cancer cell lysates and serum samples from patients diagnosed with autoimmune diseases and cancer. The obtained results agreed with those found using ELISA methodologies. Mikrochim Acta. 2022;189(4):143. 10.1007/s00604-022-05250-4 1436-5073 Mikrochimica Acta 35286499 http://hdl.handle.net/20.500.12105/14131 APRIL Amperometry BAFF CRC Dual immunoplatform MoS2/MWCNTs SLE Serum Binary MoS2 nanostructures as nanocarriers for amplification in multiplexed electrochemical immunosensing: simultaneous determination of B cell activation factor and proliferation-induced signal immunity-related cytokines.