2026-05-22T01:43:21Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/141252025-04-29T10:34:23Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19616
Repisalud
author
Melero-Fernández de Mera, Raquel María
author
Villaseñor, Alma
author
Rojo, David
author
Carrión-Navarro, Josefa
author
Gradillas, Ana
author
Ayuso-Sacido, Angel
author
Barbas, Coral
funder
Ministerio de Ciencia, Innovación y Universidades (España)
funder
Instituto de Salud Carlos III
funder
Ministerio de Economía y Competitividad (España)
funder
Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF)
2022-04-20T09:28:12Z
2022-04-20T09:28:12Z
2022-01-21
Front Oncol. 2022;11:788100.
10.3389/fonc.2021.788100
2234-943X
Frontiers in Oncology
35127492
http://hdl.handle.net/20.500.12105/14125
Glioblastoma (GBM) is one of the most malignant central nervous system tumor types. Comparative analysis of GBM tissues has rendered four major molecular subtypes. From them, two molecular subtypes are mainly found in their glioblastoma cancer stem-like cells (GSCs) derived in vitro: proneural (PN) and mesenchymal (MES) with nodular (MES-N) and semi-nodular (MES-SN) disseminations, which exhibit different metabolic, growth, and malignancy properties. Many studies suggest that cancer cells communicate between them, and the surrounding microenvironment, via exosomes. Identifying molecular markers that allow the specific isolation of GSC-derived exosomes is key in the development of new therapies. However, the differential exosome composition produced by main GSCs remains unknown. The aim of this study was to determine ceramide (Cer) composition, one of the critical lipids in both cells and their cell-derived exosomes, from the main three GSC phenotypes using mass spectrometry-based lipidomics. GSCs from human tissue samples and their cell-derived exosomes were measured using ultra-high-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UHPLC/Q-TOF-MS) in an untargeted analysis. Complete characterization of the ceramide profile, in both cells and cell-derived exosomes from GSC phenotypes, showed differential distributions among them. Results indicate that such differences of ceramide are chain-length dependent. Significant changes for the C16 Cer and C24:1 Cer and their ratio were observed among GSC phenotypes, being different for cells and their cell-derived exosomes.
eng
LC-MS
Cancer stem cells
Ceramides
Exosomes
Glioblastoma
Mesenchymal Phenotype
Proneural phenotype
Untargeted lipidomics
Ceramide Composition in Exosomes for Characterization of Glioblastoma Stem-Like Cell Phenotypes.
research article
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CeramideCompositionExosomesCharacterization_2022.pdf
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https://repisalud.isciii.es/bitstreams/754b8515-68df-4899-8c67-2d866b1be44a/download
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48c35f4ed590333e552aef0981bb15d8
78651
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CeramideCompositionExosomesCharacterization_2022.pdf.txt