2026-05-21T23:16:06Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/141252025-04-29T10:34:23Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19616

00925njm 22002777a 4500 dc Melero-Fernández de Mera, Raquel María author Villaseñor, Alma author Rojo, David author Carrión-Navarro, Josefa author Gradillas, Ana author Ayuso-Sacido, Angel author Barbas, Coral author 2022-01-21 Glioblastoma (GBM) is one of the most malignant central nervous system tumor types. Comparative analysis of GBM tissues has rendered four major molecular subtypes. From them, two molecular subtypes are mainly found in their glioblastoma cancer stem-like cells (GSCs) derived in vitro: proneural (PN) and mesenchymal (MES) with nodular (MES-N) and semi-nodular (MES-SN) disseminations, which exhibit different metabolic, growth, and malignancy properties. Many studies suggest that cancer cells communicate between them, and the surrounding microenvironment, via exosomes. Identifying molecular markers that allow the specific isolation of GSC-derived exosomes is key in the development of new therapies. However, the differential exosome composition produced by main GSCs remains unknown. The aim of this study was to determine ceramide (Cer) composition, one of the critical lipids in both cells and their cell-derived exosomes, from the main three GSC phenotypes using mass spectrometry-based lipidomics. GSCs from human tissue samples and their cell-derived exosomes were measured using ultra-high-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UHPLC/Q-TOF-MS) in an untargeted analysis. Complete characterization of the ceramide profile, in both cells and cell-derived exosomes from GSC phenotypes, showed differential distributions among them. Results indicate that such differences of ceramide are chain-length dependent. Significant changes for the C16 Cer and C24:1 Cer and their ratio were observed among GSC phenotypes, being different for cells and their cell-derived exosomes. Front Oncol. 2022;11:788100. 10.3389/fonc.2021.788100 2234-943X Frontiers in Oncology 35127492 http://hdl.handle.net/20.500.12105/14125 LC-MS Cancer stem cells Ceramides Exosomes Glioblastoma Mesenchymal Phenotype Proneural phenotype Untargeted lipidomics Ceramide Composition in Exosomes for Characterization of Glioblastoma Stem-Like Cell Phenotypes.