2026-07-21T11:28:50Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/139832024-10-31T11:45:20Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609
Repisalud
author
Becerro-Recio, David
author
González-Miguel, Javier
author
Ucero, Alberto
author
Sotillo, Javier
author
Martínez-Moreno, Álvaro
author
Pérez-Arévalo, José
author
Cwiklinski, Krystyna
author
Dalton, John P
author
Siles-Lucas, Mar
funder
Unión Europea. Comisión Europea. H2020
funder
Junta de Castilla y León (España)
funder
Ministerio de Ciencia, Innovación y Universidades (España)
funder
Agencia Estatal de Investigación (España)
funder
Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF)
funder
Ministerio de Ciencia e Innovación (España)
funder
Science Foundation Ireland
funder
Unión Europea. Comisión Europea. European Research Council (ERC)
funder
University of Valencia (España)
2022-04-18T07:46:58Z
2022-04-18T07:46:58Z
2021-06-09
Pathogens. 2021;10(6):725
10.3390/pathogens10060725
2076-0817
Pathogens (Basel, Switzerland)
34207550
http://hdl.handle.net/20.500.12105/13983
Excretory/secretory products released by helminth parasites have been widely studied for their diagnostic utility, immunomodulatory properties, as well as for their use as vaccines. Due to their location at the host/parasite interface, the characterization of parasite secretions is important to unravel the molecular interactions governing the relationships between helminth parasites and their hosts. In this study, the excretory/secretory products from adult worms of the trematode Fasciola hepatica (FhES) were employed in a combination of two-dimensional electrophoresis, immunoblot and mass spectrometry, to analyze the immune response elicited in sheep during the course of an experimental infection. Ten different immunogenic proteins from FhES recognized by serum samples from infected sheep at 4, 8, and/or 12 weeks post-infection were identified. Among these, different isoforms of cathepsin L and B, peroxiredoxin, calmodulin, or glutathione S-transferase were recognized from the beginning to the end of the experimental infection, suggesting their potential role as immunomodulatory antigens. Furthermore, four FhES proteins (C2H2-type domain-containing protein, ferritin, superoxide dismutase, and globin-3) were identified for the first time as non-immunogenic proteins. These results may help to further understand host/parasite relationships in fasciolosis, and to identify potential diagnostic molecules and drug target candidates of F. hepatica.
eng
Fasciola hepatica
Excretory/secretory antigens
Immunoproteomics
Sheep
Recognition Pattern of the Fasciola hepatica Excretome/Secretome during the Course of an Experimental Infection in Sheep by 2D Immunoproteomics
journal article
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