2026-06-14T03:45:56Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/139322024-11-28T19:06:15Zcom_20.500.12105_2052com_20.500.12105_2051com_20.500.12105_15322col_20.500.12105_19609col_20.500.12105_16963col_20.500.12105_16977

00925njm 22002777a 4500 dc Sastre, Beatriz author García-García, María Luz author Cañas, José Antonio author Calvo, Cristina author Rodrigo-Muñoz, José Manuel author Casas Flecha, Inmaculada author Mahíllo-Fernández, Ignacio author Del Pozo, Victoria author 2021 Background: Recurrent wheezing (RW) is frequently developed in infants that have suffered bronchiolitis (BCH) during first months of life, but the immune mechanism underlying is not clear. The goal was to analyze the innate immune response that characterizes BCH and RW. Methods: Ninety-eight and seventy hospitalized infants with BCH or RW diagnosis, respectively, were included. Nasopharyngeal aspirate (NPA) was processed. Cellular pellet was employed to evaluate type 2 innate lymphoid cells (ILC2) by flow cytometry and mRNA expression assays by semi-quantitative real-time PCR (qRT-PCR). In supernatant, twenty-seven pro-inflammatory and immunomodulatory factors, as well as lipid mediators and nitrites, were evaluated by ELISA and Luminex. Results: Bronchiolitis patients showed higher ILC2 percentage compared with RW (P < .05). Also, ST2+ /ILC2 percentage was higher in the BCH group than in the RW group (P < .01). TLR3, IL33, IFNG, IL10, and FLG mRNA levels were significantly increased in BCH vs RW (P < .05). In supernatant, no significant differences were reached, observing similar levels of parameters linked to vascular damage, monocyte activation, and fibroblast growth. Prostaglandin E2 and cysteinyl leukotrienes C4 were evaluated; a significant difference was only found in their ratio. Conclusion: Bronchiolitis is associated with elevated nasal percentage of ILC2. This cellular population could be the key element in the differential immune response between BCH and RW which share some mechanisms such us monocyte activation, vascular damage, and fibroblast repair. Lipid mediators could play a role in the evolution of the disease later in life through innate lymphoid cells. Pediatr Allergy Immunol. 2021 ;32(1):51-59. 10.1111/pai.13317 1399-3038 Pediatric Allergy and Immunology 32628310 http://hdl.handle.net/20.500.12105/13932 Bronchiolitis Lipid mediators Recurrent wheezing Type 2 innate lymphoid cells (ILC2) Immune response Bronchiolitis and recurrent wheezing are distinguished by type 2 innate lymphoid cells and immune response