2026-06-14T02:29:50Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/138842025-04-24T18:32:37Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609

00925njm 22002777a 4500 dc Vigon-Hernandez, Lorena author Fuertes, Daniel author García-Pérez, Javier author Torres, Montserrat author Rodríguez-Mora, Sara author Mateos, Elena author Corona, Magdalena author Saez-Marín, Adolfo J. author Malo de Molina, Rosa author Navarro, Cristina author Murciano-Antón, María Aranzazu author Cervero, Miguel author Alcamí, José author García-Gutiérrez, Valentín author Planelles, Vicente author Lopez-Huertas, Maria Rosa author Coiras, Mayte author Multidisciplinary Group of Study of COVID-19 (MGS-COVID) author 2021-05 Infection by novel coronavirus SARS-CoV-2 causes different presentations of COVID-19 and some patients may progress to a critical, fatal form of the disease that requires their admission to ICU and invasive mechanical ventilation. In order to predict in advance which patients could be more susceptible to develop a critical form of COVID-19, it is essential to define the most adequate biomarkers. In this study, we analyzed several parameters related to the cellular immune response in blood samples from 109 patients with different presentations of COVID-19 who were recruited in Hospitals and Primary Healthcare Centers in Madrid, Spain, during the first pandemic peak between April and June 2020. Hospitalized patients with the most severe forms of COVID-19 showed a potent inflammatory response that was not translated into an efficient immune response. Despite the high levels of effector cytotoxic cell populations such as NK, NKT and CD8+ T cells, they displayed immune exhaustion markers and poor cytotoxic functionality against target cells infected with pseudotyped SARS-CoV-2 or cells lacking MHC class I molecules. Moreover, patients with critical COVID-19 showed low levels of the highly cytotoxic TCRγδ+ CD8+ T cell subpopulation. Conversely, CD4 count was greatly reduced in association to high levels of Tregs, low plasma IL-2 and impaired Th1 differentiation. The relative importance of these immunological parameters to predict COVID-19 severity was analyzed by Random Forest algorithm and we concluded that the most important features were related to an efficient cytotoxic response. Therefore, efforts to fight against SARS-CoV-2 infection should be focused not only to decrease the disproportionate inflammatory response, but also to elicit an efficient cytotoxic response against the infected cells and to reduce viral replication. Front Immunol. 2021 May 26;12:665329. 10.3389/fimmu.2021.665329 1664-3224 Frontiers in Immunology 34122423 http://hdl.handle.net/20.500.12105/13884 CD8 lymphocytes + COVID-19 NK and NKT cells SARS-CoV-2 Cytotoxic response Impaired Cytotoxic Response in PBMCs From Patients With COVID-19 Admitted to the ICU: Biomarkers to Predict Disease Severity