2026-06-14T02:28:47Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/138512024-09-27T20:23:06Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609
Repisalud
author
Zhang, Shun-Xian
author
Carmena, David
author
Ballesteros, Cristina
author
Yang, Chun-Li
author
Chen, Jia-Xu
author
Chu, Yan-Hong
author
Yu, Ying-Fang
author
Wu, Xiu-Ping
author
Tian, Li-Guang
author
Serrano Ferron, Emmanuel
funder
Ministerio de Economía y Competitividad (España)
funder
National Natural Science Foundation of China
funder
China Postdoctoral Science Foundation
2022-03-28T10:22:24Z
2022-03-28T10:22:24Z
2021-05
Pathogens. 2021 May 31;10(6):684.
10.3390/pathogens10060684
2076-0817
Pathogens (Basel, Switzerland)
34072858
http://hdl.handle.net/20.500.12105/13851
Cryptosporidium spp., Entamoeba histolytica, Giardia duodenalis, and Blastocystis sp. infections have been frequently reported as etiological agents for gastroenteritis, but also as common gut inhabitants in apparently healthy individuals. Between July 2016 and March 2017, stool samples (n = 507) were collected from randomly selected individuals (male/female ratio: 1.1, age range: 38-63 years) from two sentinel hospitals in Tengchong City Yunnan Province, China. Molecular (PCR and Sanger sequencing) methods were used to detect and genotype the investigated protist species. Carriage/infection rates were: Blastocystis sp. 9.5% (95% CI: 7.1-12.4%), G. duodenalis 2.2% (95% CI: 1.1-3.8%); and E. histolytica 2.0% (95% CI: 0.9-3.6%). Cryptosporidium spp. was not detected at all. Overall, 12.4% (95% CI: 9.7-15.6) of the participants harbored at least one enteric protist species. The most common coinfection was E. histolytica and Blastocystis sp. (1.0%; 95% CI: 0.3-2.2). Sequence analyses revealed that 90.9% (10/11) of the genotyped G. duodenalis isolates corresponded to the sub-assemblage AI. The remaining sequence (9.1%, 1/11) was identified as sub-assemblage BIV. Five different Blastocystis subtypes, including ST3 (43.7%, 21/48), ST1 (27.1%, 13/48), ST7 (18.8%, 9/48), ST4 (8.3%, 4/48), and ST2 (2.1%, 1/48) were identified. Statistical analyses confirmed that (i) the co-occurrence of protist infections was purely random, (ii) no associations were observed among the four protist species found, and (iii) neither their presence, individually or jointly, nor the patient's age was predictors for developing clinical symptoms associated with these infections. Overall, these protist mono- or coinfections are asymptomatic and do not follow any pattern.
eng
Coinfection
Enteric protists
China
Giardia duodenalis
Entamoeba histolytica
Cryptosporidium
Blastocystis sp.
Genotyping
Molecular diversity
Symptomatic and Asymptomatic Protist Infections in Hospital Inpatients in Southwestern China
journal article
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