2026-08-05T11:38:42Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/138212024-09-27T23:27:22Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609
Repisalud
author
Kuchinsky, Sarah C
author
Frere, Francesca
author
Heitzman-Breen, Nora
author
Golden, Jacob
author
Vazquez, Ana
author
Honaker, Christa F
author
Siegel, Paul B
author
Ciupe, Stanca M
author
LeRoith, Tanya
author
Duggal, Nisha K
funder
National Science Foundation (Estados Unidos)
funder
Virginia Polytechnic Institute and State University (Estados Unidos)
2022-03-25T12:45:28Z
2022-03-25T12:45:28Z
2021-12
Emerg Microbes Infect. 2021 Dec;10(1):725-738.
10.1080/22221751.2021.1908850
2222-1751
Emerging Microbes & Infections
33769213
http://hdl.handle.net/20.500.12105/13821
Usutu virus (USUV; family: Flaviviridae, genus: Flavivirus), is an emerging zoonotic arbovirus that causes severe neuroinvasive disease in humans and has been implicated in the loss of breeding bird populations in Europe. USUV is maintained in an enzootic cycle between ornithophilic mosquitos and wild birds. As a member of the Japanese encephalitis serocomplex, USUV is closely related to West Nile virus (WNV) and St. Louis encephalitis virus (SLEV), both neuroinvasive arboviruses endemic in wild bird populations in the United States. An avian model for USUV is essential to understanding zoonotic transmission. Here we describe the first avian models of USUV infection with the development of viremia. Juvenile commercial ISA Brown chickens were susceptible to infection by multiple USUV strains with evidence of cardiac lesions. Juvenile chickens from two chicken lines selected for high (HAS) or low (LAS) antibody production against sheep red blood cells showed markedly different responses to USUV infection. Morbidity and mortality were observed in the LAS chickens, but not HAS chickens. LAS chickens had significantly higher viral titers in blood and other tissues, as well as oral secretions, and significantly lower development of neutralizing antibody responses compared to HAS chickens. Mathematical modelling of virus-host interactions showed that the viral clearance rate is a stronger mitigating factor for USUV viremia than neutralizing antibody response in this avian model. These chicken models provide a tool for further understanding USUV pathogenesis in birds and evaluating transmission dynamics between avian hosts and mosquito vectors.
eng
Usutu virus
Avian model
Juvenile chicken
Pathogenesis
Virus-host interactions
Pathogenesis and shedding of Usutu virus in juvenile chickens
journal article
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URL
https://repisalud.isciii.es/bitstreams/f8d7b936-b7e7-4340-b76c-7eaa439a8ff2/download
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PathogenesisAndSheddingOf_2021.pdf
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https://repisalud.isciii.es/bitstreams/96abbc95-2162-4086-8a26-975f4083c6a3/download
File
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PathogenesisAndSheddingOf_2021.pdf.txt