2026-07-23T23:02:10Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/137252024-09-27T23:39:39Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609
Repisalud
author
Thi Phung, Luyen
author
Chaiyadet, Sujittra
author
Hongsrichan, Nuttanan
author
Sotillo, Javier
author
Dinh Thi Dieu, Hang
author
Quang Tran, Canh
author
Brindley, Paul J
author
Loukas, Alex
author
Laha, Thewarach
funder
Khon Kaen University (Tailandia)
funder
National Health and Medical Research Council (Australia)
funder
NIH - National Cancer Institute (NCI) (Estados Unidos)
2022-03-04T12:27:41Z
2022-03-04T12:27:41Z
2020-04
Acta Trop. 2020 Apr;204:105355.
10.1016/j.actatropica.2020.105355
1873-6254
0001-706X
Acta Tropica
31991114
http://hdl.handle.net/20.500.12105/13725
Opisthorchiasis is a serious public health problem in East Asia and Europe. The pathology involves hepatobiliary abnormalities such as cholangitis, choledocholithiasis and tissue fibrosis that can develop into cholangiocarcinoma. Prevention of infection is difficult as multiple social and behavioral factors are involved, thus, progress on a prophylactic vaccine against opisthorchiasis is urgently needed. Opisthorchis viverrini tetraspanin-2 (Ov-TSP-2) was previously described as a potential vaccine candidate conferring partial protection against O. viverrini infections in hamsters. In this study, we generated a recombinant chimeric form of the large extracellular loop of Ov-TSP-2 and O. viverrini leucine aminopeptidase, designated rOv-TSP-2-LAP. Hamsters were vaccinated with 100 and 200 µg of rOv-TSP-2-LAP formulated with alum-CpG adjuvant via intraperitoneal injection and evaluated the level of protection against O. viverrini infection. Our results demonstrated that the number of worms recovered from hamsters vaccinated with either 100 or 200 µg of rOv-TSP-2-LAP were significantly reduced by 27% compared to the adjuvant control group. Furthermore, the average length of worms recovered from animals vaccinated with 200 μg of rOv-TSP-2-LAP was significantly shorter than those from the control adjuvant group. Immunized hamsters showed significantly increased serum levels of anti-rOv-TSP-2 IgG and IgG1 compared to adjuvant control group, suggesting that rOv-TSP-2-LAP vaccination induces a mixed Th1/Th2 immune response in hamsters. Therefore, the development of a suitable vaccine against opisthorchiasis requires further work involving new vaccine technologies to improve immunogenicity and protective efficacy.
eng
Chimeric tetraspanin-leucine aminopeptidase
Opisthorchis viverrini
Th1/th2 response
Vaccination
Partial protection with a chimeric tetraspanin-leucine aminopeptidase subunit vaccine against Opisthorchis viverrini infection in hamsters
research article
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URL
https://repisalud.isciii.es/bitstreams/dc218fe1-f340-458e-a849-3cf0e2bcd05e/download
File
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application/pdf
PartialProtectionWithA_2020.pdf
URL
https://repisalud.isciii.es/bitstreams/978571b6-2ffb-468e-80ff-9598d5658e75/download
File
MD5
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text/plain
PartialProtectionWithA_2020.pdf.txt