2026-04-29T02:37:42Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/133882025-06-03T17:49:18Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19617
Repisalud
author
Bernabeu-Zornoza, Adela
author
Coronel Lopez, Raquel
author
Palmer, Charlotte
author
López-Alonso, Victoria
author
Liste-Noya, Isabel
funder
Ministerio de Ciencia e Innovación (España)
funder
Ministerio de Economía y Competitividad (España)
funder
Comunidad de Madrid (España)
2021-09-14T12:03:36Z
2021-09-14T12:03:36Z
2021-09-02
Int J Mol Sci . 2021 Sep 2;22(17):9537.
http://hdl.handle.net/20.500.12105/13388
34502444
10.3390/ijms22179537
1422-0067
International Journal of Molecular Sciences
Amyloid-β 42 peptide (Aβ1-42 (Aβ42)) is well-known for its involvement in the development of Alzheimer's disease (AD). Aβ42 accumulates and aggregates in fibers that precipitate in the form of plaques in the brain causing toxicity; however, like other forms of Aβ peptide, the role of these peptides remains unclear. Here we analyze and compare the effects of oligomeric and fibrillary Aβ42 peptide on the biology (cell death, proliferative rate, and cell fate specification) of differentiating human neural stem cells (hNS1 cell line). By using the hNS1 cells we found that, at high concentrations, oligomeric and fibrillary Aβ42 peptides provoke apoptotic cellular death and damage of DNA in these cells, but Aβ42 fibrils have the strongest effect. The data also show that both oligomeric and fibrillar Aβ42 peptides decrease cellular proliferation but Aβ42 oligomers have the greatest effect. Finally, both, oligomers and fibrils favor gliogenesis and neurogenesis in hNS1 cells, although, in this case, the effect is more prominent in oligomers. All together the findings of this study may contribute to a better understanding of the molecular mechanisms involved in the pathology of AD and to the development of human neural stem cell-based therapies for AD treatment.
eng
Alzheimer’s
Aβ peptide
Aβ42.
Cell death
Cell differentiation
Fibrils
Human NSCs
Oligomers
Oligomeric and Fibrillar Species of Aβ42 Diversely Affect Human Neural Stem Cells
research article
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OligomericAndFibrillarSpecies_2021.pdf
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OligomericAndFibrillarSpecies_2021.pdf.txt