2026-06-14T03:38:00Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/133422025-06-17T09:49:59Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19616

00925njm 22002777a 4500 dc Sreenivasan, Krishnamoorthy author Rodríguez-delaRosa, Alejandra author Kim, Johnny author Mesquita, Diana author Segalés, Jessica author Gomez-del Arco, Pablo author Espejo, Isabel author Ianni, Alessandro author Di Croce, Luciano author Relaix, Frederic author Redondo, Juan Miguel author Braun, Thomas author Serrano, Antonio L author Perdiguero, Eusebio author Muñoz-Cánoves, Pura author 2021-08-16 Regeneration of skeletal muscle requires resident stem cells called satellite cells. Here, we report that the chromatin remodeler CHD4, a member of the nucleosome remodeling and deacetylase (NuRD) repressive complex, is essential for the expansion and regenerative functions of satellite cells. We show that conditional deletion of the Chd4 gene in satellite cells results in failure to regenerate muscle after injury. This defect is principally associated with increased stem cell plasticity and lineage infidelity during the expansion of satellite cells, caused by de-repression of non-muscle-cell lineage genes in the absence of Chd4. Thus, CHD4 ensures that a transcriptional program that safeguards satellite cell identity during muscle regeneration is maintained. Given the therapeutic potential of muscle stem cells in diverse neuromuscular pathologies, CHD4 constitutes an attractive target for satellite cell-based therapies. Stem Cell Reports . 2021;S2213-6711(21)00388-X. 10.1016/j.stemcr.2021.07.022 2213-6711 Stem Cell Reports 34450038 http://hdl.handle.net/20.500.12105/13342 Chd4 NuRD Lineage maintenance Muscle stem cell Regeneration Satellite cells Skeletal muscle CHD4 ensures stem cell lineage fidelity during skeletal muscle regeneration