2026-04-27T08:02:36Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/133162024-11-28T18:51:39Zcom_20.500.12105_19604com_20.500.12105_2051com_20.500.12105_15322col_20.500.12105_19605col_20.500.12105_16963
Repisalud
author
Macias, Alvaro
author
de la Cruz, Alicia
author
Peraza, Diego A
author
Benito-Bueno, Angela de
author
Gonzalez, Teresa
author
Valenzuela, Carmen
funder
Ministerio de Ciencia e Innovación (España)
funder
Instituto de Salud Carlos III
funder
Consejo Superior de Investigaciones Científicas (España)
funder
Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF)
2021-08-26T10:53:45Z
2021-08-26T10:53:45Z
2021-01-29
Int J Mol Sci. 2021; 22(3):1336
1422-0067
http://hdl.handle.net/20.500.12105/13316
33572906
10.3390/ijms22031336
International journal of molecular sciences
KV1.5 channel function is modified by different regulatory subunits. KVβ1.3 subunits assemble with KV1.5 channels and induce a fast and incomplete inactivation. Inhibition of PKC abolishes the KVβ1.3-induced fast inactivation, decreases the amplitude of the current KV1.5-KVβ1.3 and modifies their pharmacology likely due to changes in the traffic of KV1.5-KVβ1.3 channels in a PKC-dependent manner. In order to analyze this hypothesis, HEK293 cells were transfected with KV1.5-KVβ1.3 channels, and currents were recorded by whole-cell configuration of the patch-clamp technique. The presence of KV1.5 in the membrane was analyzed by biotinylation techniques, live cell imaging and confocal microscopy approaches. PKC inhibition resulted in a decrease of 33 ± 7% of channels in the cell surface due to reduced recycling to the plasma membrane, as was confirmed by confocal microscopy. Live cell imaging indicated that PKC inhibition almost abolished the recycling of the KV1.5-KVβ1.3 channels, generating an accumulation of channels into the cytoplasm. All these results suggest that the trafficking regulation of KV1.5-KVβ1.3 channels is dependent on phosphorylation by PKC and, therefore, they could represent a clinically relevant issue, mainly in those diseases that exhibit modifications in PKC activity.
eng
KV1.5-KVβ1.3 Recycling Is PKC-Dependent.
journal article
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URL
https://repisalud.isciii.es/bitstreams/e3205a6a-f63d-412f-89f8-40b20bc03e37/download
File
MD5
d4156a645ecd914d682a4e568c55531a
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application/pdf
K(V)1_5_K(V)β1_3Recycling_2021.pdf
URL
https://repisalud.isciii.es/bitstreams/79a7d2a6-4c46-4fd3-b917-401c49b7ebfd/download
File
MD5
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text/plain
K(V)1_5_K(V)β1_3Recycling_2021.pdf.txt