2026-05-20T03:47:29Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/133132024-11-29T10:21:33Zcom_20.500.12105_15322com_20.500.12105_2051com_20.500.12105_19604col_20.500.12105_16978col_20.500.12105_19607
Repisalud
author
Gonzalez, Maria Isabel
author
Gonzalez-Arjona, Mario
author
Santos-Coquillat, Ana
author
Vaquero, Javier
author
Vazquez-Ogando, Elena
author
Molina-Iracheta, Antonio
author
Peinado Selgas, Hector
author
Desco, Manuel
author
Salinas, Beatriz
funder
Comunidad de Madrid (España)
funder
Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF)
funder
Instituto de Salud Carlos III
funder
Ministerio de Ciencia e Innovación (España)
funder
Fundación ProCNIC
2021-08-26T09:20:40Z
2021-08-26T09:20:40Z
2021-01-16
Biomedicines. 2021; 9:81
10.3390/biomedicines9010081
2227-9059
Biomedicines
33467033
http://hdl.handle.net/20.500.12105/13313
The vertiginous increase in the use of extracellular vesicles and especially exosomes for therapeutic applications highlights the necessity of advanced techniques for gaining a deeper knowledge of their pharmacological properties. Herein, we report a novel chemical approach for the robust attachment of commercial fluorescent dyes to the exosome surface with covalent binding. The applicability of the methodology was tested on milk and cancer cell-derived exosomes (from U87 and B16F10 cancer cells). We demonstrated that fluorescent labeling did not modify the original physicochemical properties of exosomes. We tested this nanoprobe in cell cultures and healthy mice to validate its use for in vitro and in vivo applications. We confirmed that these fluorescently labeled exosomes could be successfully visualized with optical imaging.
eng
Covalently Labeled Fluorescent Exosomes for In Vitro and In Vivo Applications.
journal article
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CovalentlyLabeledFluorescentExosomes_2021.pdf.txt
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