2026-07-21T11:35:03Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/133092024-09-27T09:40:28Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19605
Repisalud
author
Campanario, Silvia
author
Ramirez-Pardo, Ignacio
author
Hong, Xiaotong
author
Isern, Joan
author
Munoz-Canoves, Pura
funder
Ministerio de Asuntos Económicos y Transformación Digital (España)
funder
Unión Europea. Comisión Europea. European Research Council (ERC)
funder
Fundación La Caixa
funder
Fundación La Marató TV3
funder
Unión Europea. Comisión Europea. H2020
funder
Agència de Gestió d'Ajuts Universitaris i de Recerca (AGAUR)
2021-08-25T07:28:16Z
2021-08-25T07:28:16Z
2020-01
Front Cell Dev Biol. 2021; 8:620409
10.3389/fcell.2020.620409
2296-634X
Frontiers in cell and developmental biology
33553156
http://hdl.handle.net/20.500.12105/13309
The skeletal muscle tissue in the adult is relatively stable under normal conditions but retains a striking ability to regenerate by its resident stem cells (satellite cells). Satellite cells exist in a quiescent (G0) state; however, in response to an injury, they reenter the cell cycle and start proliferating to provide sufficient progeny to form new myofibers or undergo self-renewal and returning to quiescence. Maintenance of satellite cell quiescence and entry of satellite cells into the activation state requires autophagy, a fundamental degradative and recycling process that preserves cellular proteostasis. With aging, satellite cell regenerative capacity declines, correlating with loss of autophagy. Enhancing autophagy in aged satellite cells restores their regenerative functions, underscoring this proteostatic activity's relevance for tissue regeneration. Here we describe two strategies for assessing autophagic activity in satellite cells from GFP-LC3 reporter mice, which allows direct autophagosome labeling, or from non-transgenic (wild-type) mice, where autophagosomes can be immunostained. Treatment of GFP-LC3 or WT satellite cells with compounds that interfere with autophagosome-lysosome fusion enables measurement of autophagic activity by flow cytometry and immunofluorescence. Thus, the methods presented permit a relatively rapid assessment of autophagy in stem cells from skeletal muscle in homeostasis and in different pathological scenarios such as regeneration, aging or disease.
eng
Assessing Autophagy in Muscle Stem Cells.
journal article
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https://repisalud.isciii.es/bitstreams/4994b4e1-2d60-403d-98fc-007d748c5ae4/download
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AssessingAutophagyinMuscle_2021.pdf
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https://repisalud.isciii.es/bitstreams/5b1f2c0f-2f0b-4d75-874f-7b018ba56375/download
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text/plain
AssessingAutophagyinMuscle_2021.pdf.txt