2026-04-29T07:24:16Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/133082024-09-27T09:07:28Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19605
Repisalud
author
Cahill, Thomas J
author
Sun, Xin
author
Ravaud, Christophe
author
Villa del Campo, Cristina
author
Klaourakis, Konstantinos
author
Lupu, Irina-Elena
author
Lord, Allegra M
author
Browne, Cathy
author
Jacobsen, Sten Eirik W
author
Greaves, David R
author
Jackson, David G
author
Cowley, Sally A
author
James, William
author
Choudhury, Robin P
author
Vieira, Joaquim Miguel
author
Riley, Paul R
funder
British Heart Foundation
funder
BHF Oxbridge Centre of Regenerative Medicine
funder
Wellcome Trust
funder
BHF Intermediate Basic Science Research
funder
British Council. BIRAX (British Israel Research and Academic Exchange Partnership)
funder
Fondation Leducq
2021-08-25T07:19:36Z
2021-08-25T07:19:36Z
2021-01
Development. 2021; 148(3):dev.194563
0950-1991
http://hdl.handle.net/20.500.12105/13308
33462113
10.1242/dev.194563
1477-9129
Development (Cambridge, England)
Macrophages are components of the innate immune system with key roles in tissue inflammation and repair. It is now evident that macrophages also support organogenesis, but few studies have characterized their identity, ontogeny and function during heart development. Here, we show that the distribution and prevalence of resident macrophages in the subepicardial compartment of the developing heart coincides with the emergence of new lymphatics, and that macrophages interact closely with the nascent lymphatic capillaries. Consequently, global macrophage deficiency led to extensive vessel disruption, with mutant hearts exhibiting shortened and mis-patterned lymphatics. The origin of cardiac macrophages was linked to the yolk sac and foetal liver. Moreover, the Cx3cr1 + myeloid lineage was found to play essential functions in the remodelling of the lymphatic endothelium. Mechanistically, macrophage hyaluronan was required for lymphatic sprouting by mediating direct macrophage-lymphatic endothelial cell interactions. Together, these findings reveal insight into the role of macrophages as indispensable mediators of lymphatic growth during the development of the mammalian cardiac vasculature.
eng
Tissue-resident macrophages regulate lymphatic vessel growth and patterning in the developing heart.
journal article
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