2026-07-21T09:55:50Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/133022024-09-27T09:39:46Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19605
Repisalud
author
Andres-Delgado, Laura
author
Galardi-Castilla, Maria
author
Munch, Juliane
author
Peralta, Marina
author
Ernst, Alexander
author
Gonzalez-Rosa, Juan Manuel
author
Tessadori, Federico
author
Santamaria, Luis
author
Bakkers, Jeroen
author
Vermot, Julien
author
de la Pompa, Jose Luis
author
Mercader, Nadia
funder
Ministerio de Economía y Competitividad (España)
funder
Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF)
funder
Swiss National Science Foundation
funder
Unión Europea. Comisión Europea. H2020
funder
Unión Europea. Comisión Europea. European Research Council (ERC)
funder
Ministerio de Ciencia e Innovación (España)
funder
Fundación ProCNIC
2021-08-24T06:27:44Z
2021-08-24T06:27:44Z
2020-12
Dev Dyn. 2020; 249(12):1455-1469
10.1002/dvdy.229
1058-8388
Developmental dynamics: an official publication of the American Association of Anatomists
33103836
http://hdl.handle.net/20.500.12105/13302
The epicardium is the outer mesothelial layer of the heart. It encloses the myocardium and plays key roles in heart development and regeneration. It derives from the proepicardium (PE), cell clusters that appear in the dorsal pericardium (DP) close to the atrioventricular canal and the venous pole of the heart, and are released into the pericardial cavity. PE cells are advected around the beating heart until they attach to the myocardium. Bmp and Notch signaling influence PE formation, but it is unclear how both signaling pathways interact during this process in the zebrafish.
Here, we show that the developing PE is influenced by Notch signaling derived from the endothelium. Overexpression of the intracellular receptor of notch in the endothelium enhances bmp expression, increases the number of pSmad1/5 positive cells in the DP and PE, and enhances PE formation. On the contrary, pharmacological inhibition of Notch1 impairs PE formation. bmp2b overexpression can rescue loss of PE formation in the presence of a Notch1 inhibitor, but Notch gain-of-function could not recover PE formation in the absence of Bmp signaling.
Endothelial Notch signaling activates bmp expression in the heart tube, which in turn induces PE cluster formation from the DP layer.
eng
Notch and Bmp signaling pathways act coordinately during the formation of the proepicardium.
journal article
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URL
https://repisalud.isciii.es/bitstreams/066e8984-8387-4fc7-9e05-7b17c6fcf463/download
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application/pdf
NotchandBmpSignaling_2020.pdf
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https://repisalud.isciii.es/bitstreams/ab3d2dce-a33d-477d-abf6-4959a1a0c14d/download
File
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text/plain
NotchandBmpSignaling_2020.pdf.txt