2026-06-14T03:38:20Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/132422024-09-27T08:15:41Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19605
Repisalud
author
Martin-Cofreces, Noa B.
author
Chichon, F J
author
Calvo, Enrique
author
Torralba, Daniel
author
Bustos-Moran, Eugenio
author
Dosil, Sara G
author
Rojas-Gomez, A
author
Bonzon-Kulichenko, Elena
author
Lopez, Juan Antonio
author
Oton, J
author
Sorrentino, A
author
Zabala, J C
author
Vernos, Isabelle
author
Vazquez, Jesus
author
Valpuesta, Jose
author
Sanchez-Madrid, Francisco
funder
ALBA Synchrotron
funder
Ministerio de Economía y Competitividad (España)
funder
Comunidad de Madrid (España)
funder
Fundación Ramón Areces
funder
Fundación BBVA
funder
Fundación La Marató TV3
funder
Fundación La Caixa
funder
Ministerio de Ciencia e Innovación (España)
funder
Fundación ProCNIC
funder
Centro Nacional de Biotecnología (España)
2021-07-15T10:28:29Z
2021-07-15T10:28:29Z
2020-12
Sci Adv. 2020; 6(49):eabb7242
10.1126/sciadv.abb7242
2375-2548
Science advances
33268369
http://hdl.handle.net/20.500.12105/13242
T lymphocyte activation requires the formation of immune synapses (IS) with antigen-presenting cells. The dynamics of membrane receptors, signaling scaffolds, microfilaments, and microtubules at the IS determine the potency of T cell activation and subsequent immune response. Here, we show that the cytosolic chaperonin CCT (chaperonin-containing TCP1) controls the changes in reciprocal orientation of the centrioles and polarization of the tubulin dynamics induced by T cell receptor in T lymphocytes forming an IS. CCT also controls the mitochondrial ultrastructure and the metabolic status of T cells, regulating the de novo synthesis of tubulin as well as posttranslational modifications (poly-glutamylation, acetylation, Δ1 and Δ2) of αβ-tubulin heterodimers, fine-tuning tubulin dynamics. These changes ultimately determine the function and organization of the centrioles, as shown by three-dimensional reconstruction of resting and stimulated primary T cells using cryo-soft x-ray tomography. Through this mechanism, CCT governs T cell activation and polarity.
eng
The chaperonin CCT controls T cell receptor-driven 3D configuration of centrioles.
journal article
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URL
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ThechaperoninCCTcontrols_2020.pdf
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ThechaperoninCCTcontrols_2020_supp.pdf
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ThechaperoninCCTcontrols_2020.pdf.txt
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ThechaperoninCCTcontrols_2020_supp.pdf.txt