2026-06-14T03:43:20Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/132422024-09-27T08:15:41Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19605

00925njm 22002777a 4500 dc Martin-Cofreces, Noa B. author Chichon, F J author Calvo, Enrique author Torralba, Daniel author Bustos-Moran, Eugenio author Dosil, Sara G author Rojas-Gomez, A author Bonzon-Kulichenko, Elena author Lopez, Juan Antonio author Oton, J author Sorrentino, A author Zabala, J C author Vernos, Isabelle author Vazquez, Jesus author Valpuesta, Jose author Sanchez-Madrid, Francisco author 2020-12 T lymphocyte activation requires the formation of immune synapses (IS) with antigen-presenting cells. The dynamics of membrane receptors, signaling scaffolds, microfilaments, and microtubules at the IS determine the potency of T cell activation and subsequent immune response. Here, we show that the cytosolic chaperonin CCT (chaperonin-containing TCP1) controls the changes in reciprocal orientation of the centrioles and polarization of the tubulin dynamics induced by T cell receptor in T lymphocytes forming an IS. CCT also controls the mitochondrial ultrastructure and the metabolic status of T cells, regulating the de novo synthesis of tubulin as well as posttranslational modifications (poly-glutamylation, acetylation, Δ1 and Δ2) of αβ-tubulin heterodimers, fine-tuning tubulin dynamics. These changes ultimately determine the function and organization of the centrioles, as shown by three-dimensional reconstruction of resting and stimulated primary T cells using cryo-soft x-ray tomography. Through this mechanism, CCT governs T cell activation and polarity. Sci Adv. 2020; 6(49):eabb7242 10.1126/sciadv.abb7242 2375-2548 Science advances 33268369 http://hdl.handle.net/20.500.12105/13242 The chaperonin CCT controls T cell receptor-driven 3D configuration of centrioles.