2026-06-14T03:43:01Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/131722024-09-27T10:04:55Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19607
Repisalud
author
Gomez-Gaviro, Maria Victoria
author
Sanderson, Daniel
author
Ripoll, Jorge
author
Desco, Manuel
funder
Ministerio de Ciencia, Innovación y Universidades (España)
funder
Instituto de Salud Carlos III
funder
Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF)
funder
Fundación ProCNIC
funder
Centro de Investigación Biomedica en Red - CIBER
2021-06-22T08:31:01Z
2021-06-22T08:31:01Z
2020-08-21
iScience. 2020; 23(8):101432
10.1016/j.isci.2020.101432
2589-0042
iScience
32805648
http://hdl.handle.net/20.500.12105/13172
Three-dimensional (3D) optical imaging techniques can expand our knowledge about physiological and pathological processes that cannot be fully understood with 2D approaches. Standard diagnostic tests frequently are not sufficient to unequivocally determine the presence of a pathological condition. Whole-organ optical imaging requires tissue transparency, which can be achieved by using tissue clearing procedures enabling deeper image acquisition and therefore making possible the analysis of large-scale biological tissue samples. Here, we review currently available clearing agents, methods, and their application in imaging of physiological or pathological conditions in different animal and human organs. We also compare different optical tissue clearing methods discussing their advantages and disadvantages and review the use of different 3D imaging techniques for the visualization and image acquisition of cleared tissues. The use of optical tissue clearing resources for large-scale biological tissues 3D imaging paves the way for future applications in translational and clinical research.
eng
Biomedical Applications of Tissue Clearing and Three-Dimensional Imaging in Health and Disease.
journal article
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URL
https://repisalud.isciii.es/bitstreams/881bde3e-fe31-43e4-a04b-194e3aff0ea7/download
File
MD5
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application/pdf
BiomedicalApplicationsofTissue_2020.pdf
URL
https://repisalud.isciii.es/bitstreams/e71242ca-bf30-4ff4-b8fe-eb5d7230f05d/download
File
MD5
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text/plain
BiomedicalApplicationsofTissue_2020.pdf.txt