2026-06-30T03:57:09Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/129762025-05-05T18:46:03Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609
Repisalud
author
Verburg, Ilse
author
van Veelen, H Pieter J
author
Waar, Karola
author
Rossen, John W A
author
Friedrich, Alex W
author
Hernández Leal, Lucia
author
García-Cobos, Silvia
author
Schmitt, Heike
funder
Dutch Ministry of Economic Affairs
funder
Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF)
funder
Interreg V-A España-Portugal
funder
Dutch Ministry of Infrastructure and Environment
2021-05-18T07:41:08Z
2021-05-18T07:41:08Z
2021-03-31
Microorganisms. 2021 Mar 31;9(4):718.
10.3390/microorganisms9040718
2076-2607
Microorganisms
33807193
http://hdl.handle.net/20.500.12105/12976
This study pertains to measure differences in bacterial communities along the wastewater pathway, from sewage sources through the environment. Our main focus was on taxa which include pathogenic genera, and genera harboring antibiotic resistance (henceforth referred to as "target taxa"). Our objective was to measure the relative abundance of these taxa in clinical wastewaters compared to non-clinical wastewaters, and to investigate what changes can be detected along the wastewater pathway. The study entailed a monthly sampling campaign along a wastewater pathway, and taxa identification through 16S rRNA amplicon sequencing. Results indicated that clinical and non-clinical wastewaters differed in their overall bacterial composition, but that target taxa were not enriched in clinical wastewater. This suggests that treatment of clinical wastewater before release into the wastewater system would only remove a minor part of the potential total pathogen load in wastewater treatment plants. Additional findings were that the relative abundance of most target taxa was decreased after wastewater treatment, yet all investigated taxa were detected in 68% of the treated effluent samples-meaning that these bacteria are continuously released into the receiving surface water. Temporal variation was only observed for specific taxa in surface water, but not in wastewater samples.
eng
16S rRNA amplicon sequencing
Bacterial community structure
Clinical wastewater
Sampling campaign
Effects of Clinical Wastewater on the Bacterial Community Structure from Sewage to the Environment
research article
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