2026-06-30T03:03:41Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/129762025-05-05T18:46:03Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609

00925njm 22002777a 4500 dc Verburg, Ilse author van Veelen, H Pieter J author Waar, Karola author Rossen, John W A author Friedrich, Alex W author Hernández Leal, Lucia author García-Cobos, Silvia author Schmitt, Heike author 2021-03-31 This study pertains to measure differences in bacterial communities along the wastewater pathway, from sewage sources through the environment. Our main focus was on taxa which include pathogenic genera, and genera harboring antibiotic resistance (henceforth referred to as "target taxa"). Our objective was to measure the relative abundance of these taxa in clinical wastewaters compared to non-clinical wastewaters, and to investigate what changes can be detected along the wastewater pathway. The study entailed a monthly sampling campaign along a wastewater pathway, and taxa identification through 16S rRNA amplicon sequencing. Results indicated that clinical and non-clinical wastewaters differed in their overall bacterial composition, but that target taxa were not enriched in clinical wastewater. This suggests that treatment of clinical wastewater before release into the wastewater system would only remove a minor part of the potential total pathogen load in wastewater treatment plants. Additional findings were that the relative abundance of most target taxa was decreased after wastewater treatment, yet all investigated taxa were detected in 68% of the treated effluent samples-meaning that these bacteria are continuously released into the receiving surface water. Temporal variation was only observed for specific taxa in surface water, but not in wastewater samples. Microorganisms. 2021 Mar 31;9(4):718. 10.3390/microorganisms9040718 2076-2607 Microorganisms 33807193 http://hdl.handle.net/20.500.12105/12976 16S rRNA amplicon sequencing Bacterial community structure Clinical wastewater Sampling campaign Effects of Clinical Wastewater on the Bacterial Community Structure from Sewage to the Environment