2026-06-14T05:07:02Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/129552025-05-08T18:13:06Zcom_20.500.12105_2052com_20.500.12105_2051com_20.500.12105_15322col_20.500.12105_19609col_20.500.12105_16990
Repisalud
author
García-Suárez, María Del Mar
author
González-Rodríguez, Irene
author
Cima-Cabal, María Dolores
author
Yuste, Jose Enrique
author
Vázquez, Fernando
author
Santiago, Enrique
funder
University of Oviedo (España)
funder
Ministerio de Educación, Cultura y Deporte (España)
funder
Universidad Internacional de La Rioja (España)
2021-05-13T14:58:08Z
2021-05-13T14:58:08Z
2019-11-18
Diagnostics (Basel). 2019; 9(4):196
10.3390/diagnostics9040196
2075-4418
Diagnostics (Basel)
31752204
http://hdl.handle.net/20.500.12105/12955
Streptococcus pneumoniae shows more than 90 capsular serotypes that can be distinguished by their reactivity against antisera. The main objective of this work was the development of a molecular method for serotyping without the use of antisera. A computer program containing an algorithm was used to search in a database for potentially useful enzymes for Restriction Fragment Length Polymorphism-RFLP typing, in order to maximize the discrimination between different serotypes. DNA sequences of 90 serotypes for the region between dexB and aliA genes were compiled, and a computer screening of restriction enzymes was performed. The wzg-wzh-wzd-wze region and Sse9I restriction predicted unique PCR-RFLP patterns for 39 serotypes and eight serogroups. A second restriction enzyme resolved fragment specific patterns for 25 serotypes. The method was tested with 98 serotype-unknown clinical isolates. PCR-RFLP analysis deduced correct serotypes that were confirmed by Quellung reaction for 78.5% of the isolates.
eng
PCR-RFLP
Streptococcus pneumoniae
Serotype
Identification of Pneumococcal Serotypes by PCR-Restriction Fragment Length Polymorphism.
research article
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URL
https://repisalud.isciii.es/bitstreams/3b17832c-f204-4dfd-8656-872cc85b59de/download
File
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application/pdf
IdentificationOfPneumococcalSerotypes_2019.pdf
URL
https://repisalud.isciii.es/bitstreams/05abe728-81a1-44dc-86b8-c64c62c2f51f/download
File
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text/plain
IdentificationOfPneumococcalSerotypes_2019.pdf.txt