2026-06-14T05:17:39Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/129552025-05-08T18:13:06Zcom_20.500.12105_2052com_20.500.12105_2051com_20.500.12105_15322col_20.500.12105_19609col_20.500.12105_16990

00925njm 22002777a 4500 dc García-Suárez, María Del Mar author González-Rodríguez, Irene author Cima-Cabal, María Dolores author Yuste, Jose Enrique author Vázquez, Fernando author Santiago, Enrique author 2019-11-18 Streptococcus pneumoniae shows more than 90 capsular serotypes that can be distinguished by their reactivity against antisera. The main objective of this work was the development of a molecular method for serotyping without the use of antisera. A computer program containing an algorithm was used to search in a database for potentially useful enzymes for Restriction Fragment Length Polymorphism-RFLP typing, in order to maximize the discrimination between different serotypes. DNA sequences of 90 serotypes for the region between dexB and aliA genes were compiled, and a computer screening of restriction enzymes was performed. The wzg-wzh-wzd-wze region and Sse9I restriction predicted unique PCR-RFLP patterns for 39 serotypes and eight serogroups. A second restriction enzyme resolved fragment specific patterns for 25 serotypes. The method was tested with 98 serotype-unknown clinical isolates. PCR-RFLP analysis deduced correct serotypes that were confirmed by Quellung reaction for 78.5% of the isolates. Diagnostics (Basel). 2019; 9(4):196 10.3390/diagnostics9040196 2075-4418 Diagnostics (Basel) 31752204 http://hdl.handle.net/20.500.12105/12955 PCR-RFLP Streptococcus pneumoniae Serotype Identification of Pneumococcal Serotypes by PCR-Restriction Fragment Length Polymorphism.