2026-07-21T14:57:52Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/129462025-06-13T09:42:53Zcom_20.500.12105_19604com_20.500.12105_2051com_20.500.12105_2052col_20.500.12105_19605col_20.500.12105_19617

00925njm 22002777a 4500 dc Prieto, Ignacio author Zambrano, Alberto author Laso, Javier author Aranda, Ana author Samper, Enrique author Monsalve, María author 2019 Aims: Oxidative stress is known to induce early replicative senescence. Senescence has been proposed to work as a barrier to immortalization and tumor development. Here, we aimed to evaluate the impact of the loss of peroxisome proliferator activated receptor γ co-activator 1α (PGC-1α), a master regulator of oxidative metabolism and mitochondrial reactive oxygen species (ROS) generation, on replicative senescence and immortalization in mouse embryonic fibroblasts (MEFs). Results: We found that primary MEFs lacking PGC-1α showed higher levels of ROS than wild-type MEFs at all cell passages tested. The elevated production of ROS was associated with higher levels of oxidative DNA damage and the increased formation of DNA double-strand breaks. Evaluation of the induction of DNA repair systems in response to γ-radiation indicated that the loss of PGC-1α also resulted in a small but significant reduction in their activity. DNA damage induced the early activation of senescence markers, including an increase in the number of β-galactosidase-positive cells, the induction of p53 phosphorylation, and the increase in p16 and p19 protein. These changes were, however, not sufficient to reduce proliferation rates of PGC-1α-deficient MEFs at any cell passage tested. Moreover, PGC-1α-deficient cells escaped replicative senescence. Innovation & conclusion: PGC-1α plays an important role in the control of cellular senescence and immortalization. Free Radic Biol Med. 2019 Jul;138:23-32. 10.1016/j.freeradbiomed.2019.04.015 1873-4596 Free Radical Biology & Medicine 31029787 http://hdl.handle.net/20.500.12105/12946 Immortalization Mitochondria Oxidative stress PGC-1α Senescence Early induction of senescence and immortalization in PGC-1α-deficient mouse embryonic fibroblasts