2026-07-16T12:40:42Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/128892024-10-31T11:50:45Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609
Repisalud
author
Messa, Augusto
author
Köster, Pamela Carolina
author
Garrine, Marcelino
author
Nhampossa, Tacilta
author
Massora, Sérgio
author
Cossa, Anélsio
author
Bassat, Quique
author
Kotloff, Karen
author
Levine, Myron M
author
Alonso, Pedro L
author
Carmena, David
author
Mandomando, Inácio
funder
Bill & Melinda Gates Foundation
funder
Instituto de Salud Carlos III
funder
Ministerio de Economía y Competitividad (España)
funder
Ministry of Science and Technology (Mozambique)
funder
USAID Country Office of Mozambique
2021-05-06T09:15:59Z
2021-05-06T09:15:59Z
2021-04-09
Pathogens. 2021 Apr 9;10(4):452.
10.3390/pathogens10040452
2076-0817
Pathogens (Basel, Switzerland)
33918893
http://hdl.handle.net/20.500.12105/12889
Cryptosporidium is a leading cause of childhood diarrhoea and associated physical and cognitive impairment in low-resource settings. Cryptosporidium-positive faecal samples (n = 190) from children aged ≤ 5 years enrolled in the Global Enteric Multicenter Study (GEMS) in Mozambique detected by ELISA (11.5%, 430/3754) were successfully PCR-amplified and sequenced at the gp60 or ssu rRNA loci for species determination and genotyping. Three Cryptosporidium species including C. hominis (72.6%, 138/190), C. parvum (22.6%, 43/190), and C. meleagridis (4.2%, 8/190) were detected. Children ≤ 23 months were more exposed to Cryptosporidium spp. infections than older children. Both C. hominis and C. parvum were more prevalent among children with diarrhoeal disease compared to those children without it (47.6% vs. 33.3%, p = 0.007 and 23.7% vs. 11.8%, p = 0.014, respectively). A high intra-species genetic variability was observed within C. hominis (subtype families Ia, Ib, Id, Ie, and If) and C. parvum (subtype families IIb, IIc, IIe, and IIi) but not within C. meleagridis (subtype family IIIb). No association between Cryptosporidium species/genotypes and child's age was demonstrated. The predominance of C. hominis and C. parvum IIc suggests that most of the Cryptosporidium infections were anthroponotically transmitted, although zoonotic transmission events also occurred at an unknown rate. The role of livestock, poultry, and other domestic animal species as sources of environmental contamination and human cryptosporidiosis should be investigated in further molecular epidemiological studies in Mozambique.
eng
Cryptosporidium
GEMS
Mozambique
Children
Diarrhoea
Genotyping
gp60
Molecular epidemiology
Prevalence
ssu rRNA
Molecular Characterisation of Cryptosporidium spp. in Mozambican Children Younger than 5 Years Enrolled in a Matched Case-Control Study on the Aetiology of Diarrhoeal Disease.
journal article
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