2026-04-29T06:16:20Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/128842024-09-27T20:30:01Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609
Repisalud
author
Bernal-Martinez, Leticia
author
Herrera-Leon, Laura
author
Valero, Clara
author
de la Cruz, Paula
author
Ghimpu, Larisa
author
Mesa-Arango, Ana Cecilia
author
Santoni, Gabriela
author
Goterris, Lidia
author
Millán, Rosario
author
Buitrago, Maria Jose
funder
Instituto de Salud Carlos III
funder
Ministerio de Ciencia e Innovación (España)
funder
Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF)
funder
RETICS-Investigación en Patología Infecciosa (REIPI-ISCIII) (España)
funder
Fundación Carolina
2021-05-05T19:18:00Z
2021-05-05T19:18:00Z
2021-04-27
J Fungi (Basel) . 2021 Apr 27;7(5):336.
http://hdl.handle.net/20.500.12105/12884
33925404
10.3390/jof7050336
2309-608X
Journal of Fungi (Basel, Switzerland)
Opportunistic fungal pneumonias (OFP) are the main cause of death in AIDS patients worldwide. Diagnosis of these infections is often late as tuberculosis (TB) is frequently the first suspicion. In addition, diagnostic tools have limitations and are unavailable in disadvantaged regions. To perform the differential diagnosis of the main fungi causing OFP in AIDS patients (Histoplasma capsulatum, Cryptococcus neoformans/C. gattii and Pneumocystis jirovecii) vs. the Mycobacterium tuberculosis complex (MTBC), two new assays were developed: (i) a multiplex real-time PCR (MRT-PCR) and (ii) a simple and cost-effective method based on real-time PCR and the analysis of melting curves after amplification (MC-PCR). Both of the techniques were optimized and standardized "in vitro", showing a suitable reproducibility (CV ranged between 1.84 and 3.81% and 1.41 and 4.83%, respectively), a 100% specificity and detection limits between 20 and 2 fg of genomic DNA per 20 µL of reaction. A validation study was performed by retrospectively using 42 clinical samples from 37 patients with proven fungal infection or TB, and 33 controls. The overall sensitivity for the MRT-PCR assay and the MC-PCR assay was 88% and 90.4%, respectively. Both techniques were fast, sensitive and reproducible, allowing for the detection of these pathogens and the performance of a differential diagnosis.
eng
Differential diagnosis
Fungal pneumonia
Tuberculosis
Differential Diagnosis of Fungal Pneumonias vs. Tuberculosis in AIDS Patients by Using Two New Molecular Methods.
journal article
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DifferentialDiagnosisOfFungal_2021.pdf.txt
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