2026-06-14T02:28:44Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/127072024-09-27T20:50:29Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609col_20.500.12105_19613
Repisalud
author
Parker, Lucy A
author
Acosta, Lucrecia
author
Gutierrez, Mariana Noel
author
Cruz, Israel
author
Nieto Martinez, Francisco Javier
author
Deschutter, Enrique Jorge
author
Bornay-Llinares, Fernando Jorge
funder
Unión Europea. Comisión Europea. 6 Programa Marco
funder
Generalitat Valenciana (España)
2021-04-20T15:02:52Z
2021-04-20T15:02:52Z
2021
Front Vet Sci . 2021 Mar 8;8:642287
10.3389/fvets.2021.642287
2297-1769
Frontiers in veterinary science
33763466
http://hdl.handle.net/20.500.12105/12707
Background: Visceral leishmaniasis (VL) is one of the most important parasitic diseases in the world. The domestic dog is the main reservoir of zoonotic VL and a high prevalence of canine leishmaniasis (CanL) is associated with transmission of infection to humans. Here we describe the methodology used to obtain a rapid and representative sample of domestic dogs in the city of Posadas, Misiones, and compare the prevalence of Leishmania infection with a sample of shelter dogs. Methodology: We used the city land registry to make a random selection of homes and systematically recruited 349 domestic dogs from the selected properties. We also included all dogs from the main canine shelter within the city. Dogs were examined by two experienced veterinarians who recorded the presence of clinical signs common in CanL using a standardized protocol. We extracted a blood sample from each dog and performed four different serological tests to reveal the presence of anti-Leishmania antibodies. Results: After clinical examination, 145 domestic dogs (41.5%) and 63 (90%) shelter dogs had clinical signs compatible with CanL (p < 0.001). The seroprevalence among domestic dogs was 20.1% (95% CI 16.1-24.6) which was significantly lower than among the abandoned dogs (38.6%, 95% CI 27.7-50.6, p < 0.001). The spatial distribution of infected dogs was fairly homogenous throughout the city. Among domestic dogs, we observed a positive association between where the dog slept and presence of anti-Leishmania antibodies (p = 0.034). Of the seropositive domestic dogs 38 (54.4%) were asymptomatic. Conclusions: Our findings demonstrate how seroprevalence results can be highly influenced by sampling methodology. We demonstrate how the land registry can be used to estimate the prevalence of CanL in representative sample of domestic dogs in an urban setting, allowing decision makers to deepen their understanding the epidemiology of CanL in a timely and efficient manner for the development of plans to address both human and canine disease.
eng
Visceral leishmaniasis
Canine leishmaniasis
Sampling
Epidemiology
Canine population
A Novel Sampling Model to Study the Epidemiology of Canine Leishmaniasis in an Urban Environment.
research article
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