2026-06-14T03:45:12Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/126382025-05-07T12:31:14Zcom_20.500.12105_15322com_20.500.12105_2051com_20.500.12105_2052col_20.500.12105_16963col_20.500.12105_19617
Repisalud
author
López-Janeiro, Álvaro
author
Ruz-Caracuel, Ignacio
author
Ramón-Patino, Jorge L
author
de los Ríos, Vivian
author
Villalba Esparza, María
author
Berjón, Alberto
author
Yébenes, Laura
author
Hernández, Alicia
author
Masetto, Ivan
author
Kadioglu, Ece
author
Goubert, Virginie
author
Heredia-Soto, Victoria
author
Barderas Manchado, Rodrigo
author
Casal, José Ignacio
author
de Andrea, Carlos E
author
Redondo, Andres
author
Mendiola, Marta
author
Peláez-García, Alberto
author
Hardisson, David
funder
Instituto de Salud Carlos III
funder
Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF)
2021-04-13T18:22:06Z
2021-04-13T18:22:06Z
2021-02-14
Cancers (Basel) . 2021 Feb 14;13(4):794.
10.3390/cancers13040794
2072-6694
Cancers
33672863
http://hdl.handle.net/20.500.12105/12638
Low-grade, early-stage endometrial carcinoma (EC) is the most frequent malignant tumor of the uterine corpus. However, the molecular alterations that underlie these tumors are far from being fully understood. The purpose of this study is to describe dysregulated molecular pathways from EC patients. Sixteen samples of tumor tissue and paired healthy controls were collected and both were subjected to mass spectrometry (MS)/MS proteomic analysis. Gene ontology and pathway analysis was performed to discover dysregulated pathways and/or proteins using different databases and bioinformatic tools. Dysregulated pathways were cross-validated in an independent external cohort. Cell signaling, immune response, and cell death-associated pathways were robustly identified. The SLIT/ROBO signaling pathway demonstrated dysregulation at the proteomic and transcriptomic level. Necroptosis and ferroptosis were cell death-associated processes aberrantly regulated, in addition to apoptosis. Immune response-associated pathways showed a dominance of innate immune responses. Tumor immune infiltrates measured by immunofluorescence demonstrated diverse lymphoid and myeloid populations. Our results suggest a role of SLIT/ROBO, necroptosis, and ferroptosis, as well as a prominent role of innate immune response in low-grade, early-stage EC. These results could guide future research in this group of tumors.
eng
SLIT/ROBO
Endometrial cancer
Ferroptosis
Immune microenvironment
Low grade
Necroptosis
Pathways
Proteomics
Proteomic Analysis of Low-Grade, Early-Stage Endometrial Carcinoma Reveals New Dysregulated Pathways Associated with Cell Death and Cell Signaling.
research article
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ProteomicAnalysisOfLow_2021.pdf
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https://repisalud.isciii.es/bitstreams/029f9229-04b1-455b-af44-404d38aae36c/download
File
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text/plain
ProteomicAnalysisOfLow_2021.pdf.txt