2026-06-14T03:54:57Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/126152024-09-27T19:19:57Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609
Repisalud
author
Köster, Pamela Carolina
author
Malheiros, Antonio F
author
Shaw, Jeffrey J
author
Balasegaram, Sooria
author
Prendergast, Alexander
author
Lucaccioni, Héloïse
author
Moreira, Luciana Melhorança
author
Lemos, Larissa M S
author
Dashti, Alejandro
author
Bailo-Barroso, Begoña
author
Marcili, Arlei
author
Sousa Soares, Herbert
author
Gennari, Solange Maria
author
Calero-Bernal, Rafael
author
Gonzalez-Barrio, David
author
Carmena, David
funder
São Paulo Research Foundation
funder
National Health Foundation (FUNASA, Brazil)
funder
Mato Grosso State Research Support Foundation (FAPEMAT, Brazil)
funder
Instituto de Salud Carlos III
funder
Ministerio de Economía y Competitividad (España)
2021-04-12T20:48:22Z
2021-04-12T20:48:22Z
2021-02-14
Pathogens . 2021 Feb 14;10(2):206.
10.3390/pathogens10020206
2076-0817
Pathogens (Basel, Switzerland)
33672794
http://hdl.handle.net/20.500.12105/12615
Little information is available on the occurrence and genetic variability of the diarrhoea-causing enteric protozoan parasite Giardia duodenalis in indigenous communities in Brazil. This cross-sectional epidemiological survey describes the frequency, genotypes, and risk associations for this pathogen in Tapirapé people (Brazilian Amazon) at four sampling campaigns during 2008-2009. Microscopy was used as a screening test, and molecular (PCR and Sanger sequencing) assays targeting the small subunit ribosomal RNA, the glutamate dehydrogenase, the beta-giardin, and the triosephosphate isomerase genes as confirmatory/genotyping methods. Associations between G. duodenalis and sociodemographic and clinical variables were investigated using Chi-squared test and univariable/multivariable logistic regression models. Overall, 574 individuals belonging to six tribes participated in the study, with G. duodenalis prevalence rates varying from 13.5-21.7%. The infection was positively linked to younger age and tribe. Infected children <15 years old reported more frequent gastrointestinal symptoms compared to adults. Assemblage B accounted for three out of four G. duodenalis infections and showed a high genetic diversity. No association between assemblage and age or occurrence of diarrhoea was demonstrated. These data indicate that the most likely source of infection was anthropic and that different pathways (e.g., drinking water) may be involved in the transmission of the parasite.
eng
Amazon
Brazil
Giardia
Tapirapé
Asymptomatic
Community
Genotyping
Indigenous
Risk association
Transmission
Multilocus Genotyping of Giardia duodenalis in Mostly Asymptomatic Indigenous People from the Tapirapé Tribe, Brazilian Amazon.
research article
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MultilocusGenotypingOfGiardia_2021.pdf
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MultilocusGenotypingOfGiardia_2021.pdf.txt