2026-06-14T02:29:31Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/126152024-09-27T19:19:57Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609

00925njm 22002777a 4500 dc Köster, Pamela Carolina author Malheiros, Antonio F author Shaw, Jeffrey J author Balasegaram, Sooria author Prendergast, Alexander author Lucaccioni, Héloïse author Moreira, Luciana Melhorança author Lemos, Larissa M S author Dashti, Alejandro author Bailo-Barroso, Begoña author Marcili, Arlei author Sousa Soares, Herbert author Gennari, Solange Maria author Calero-Bernal, Rafael author Gonzalez-Barrio, David author Carmena, David author 2021-02-14 Little information is available on the occurrence and genetic variability of the diarrhoea-causing enteric protozoan parasite Giardia duodenalis in indigenous communities in Brazil. This cross-sectional epidemiological survey describes the frequency, genotypes, and risk associations for this pathogen in Tapirapé people (Brazilian Amazon) at four sampling campaigns during 2008-2009. Microscopy was used as a screening test, and molecular (PCR and Sanger sequencing) assays targeting the small subunit ribosomal RNA, the glutamate dehydrogenase, the beta-giardin, and the triosephosphate isomerase genes as confirmatory/genotyping methods. Associations between G. duodenalis and sociodemographic and clinical variables were investigated using Chi-squared test and univariable/multivariable logistic regression models. Overall, 574 individuals belonging to six tribes participated in the study, with G. duodenalis prevalence rates varying from 13.5-21.7%. The infection was positively linked to younger age and tribe. Infected children <15 years old reported more frequent gastrointestinal symptoms compared to adults. Assemblage B accounted for three out of four G. duodenalis infections and showed a high genetic diversity. No association between assemblage and age or occurrence of diarrhoea was demonstrated. These data indicate that the most likely source of infection was anthropic and that different pathways (e.g., drinking water) may be involved in the transmission of the parasite. Pathogens . 2021 Feb 14;10(2):206. 10.3390/pathogens10020206 2076-0817 Pathogens (Basel, Switzerland) 33672794 http://hdl.handle.net/20.500.12105/12615 Amazon Brazil Giardia Tapirapé Asymptomatic Community Genotyping Indigenous Risk association Transmission Multilocus Genotyping of Giardia duodenalis in Mostly Asymptomatic Indigenous People from the Tapirapé Tribe, Brazilian Amazon.