2026-04-29T02:37:35Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/125862025-01-08T08:30:51Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609

00925njm 22002777a 4500 dc Strauss, Ricardo A author Herrera-Leon, Laura author Guillén, Ana C author Castro, Julio S author Lorenz, Eva author Carvajal, Ana author Hernandez, Elizabeth author Navas, Trina author Vielma, Silvana author Lopez, Neiris author Lopez, Maria G author Aurenty, Lisbeth author Navas, Valeria author Rosas, Maria A author Drummond, Tatiana author Martínez, José G author Hernández, Erick author Bertuglia, Francis author Andrade, Omaira author Torres, Jaime author May, Jürgen author Herrera-León, Silvia author Eibach, Daniel author 2021-03-18 In 2016, Venezuela faced a large diphtheria outbreak that extended until 2019. Nasopharyngeal or oropharyngeal samples were prospectively collected from 51 suspected cases and retrospective data from 348 clinical records was retrieved from 14 hospitals between November 2017 and November 2018. Confirmed pathogenic Corynebactrium isolates were biotyped. Multilocus Sequence Typing (MLST) was performed followed by next-generation-based core genome-MLST and minimum spanning trees were generated. Subjects between 10 and 19 years of age were mostly affected (n = 95; 27.3%). Case fatality rates (CFR) were higher in males (19.4%), as compared to females (15.8%). The highest CFR (31.1%) was observed among those under 5, followed by the 40 to 49 age-group (25.0%). Nine samples corresponded to C. diphtheriae and 1 to C. ulcerans. Two Sequencing Types (ST), ST174 and ST697 (the latter not previously described) were identified among the eight C. diphtheriae isolates from Carabobo state. Cg-MLST revealed only one cluster also from Carabobo. The Whole Genome Sequencing analysis revealed that the outbreak seemed to be caused by different strains with C. diphtheriae and C. ulcerans coexisting. The reemergence and length of this outbreak suggest vaccination coverage problems and an inadequate control strategy. Sci Rep . 2021 Mar 18;11(1):6378. http://hdl.handle.net/20.500.12105/12586 33737710 10.1038/s41598-021-85957-1 2045-2322 Scientific reports Molecular and epidemiologic characterization of the diphtheria outbreak in Venezuela.