2026-06-14T06:44:36Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/125742024-09-27T21:31:47Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609
Repisalud
author
Muadica, Aly Salimo
author
Köster, Pamela Carolina
author
Dashti, Alejandro
author
Bailo-Barroso, Begoña
author
Hernandez-De-Mingo, Marta
author
Balasegaram, Sooria
author
Carmena, David
funder
Instituto de Salud Carlos III
2021-04-09T09:10:11Z
2021-04-09T09:10:11Z
2021-02-24
Pathogens. 2021 Feb 24;10(3):255.
10.3390/pathogens10030255
2076-0817
Pathogens (Basel, Switzerland)
33668348
http://hdl.handle.net/20.500.12105/12574
Infections by the protist enteroparasites Giardia duodenalis, Cryptosporidium spp., and, to a much lesser extent, Blastocystis sp. are common causes of childhood diarrhoea in low-income countries. This molecular epidemiological study assesses the frequency and molecular diversity of these pathogens in faecal samples from asymptomatic schoolchildren (n = 807) and symptomatic children seeking medical attention (n = 286) in Zambézia province, Mozambique. Detection and molecular characterisation of pathogens was conducted by polymerase chain reaction (PCR)-based methods coupled with Sanger sequencing. Giardia duodenalis was the most prevalent enteric parasite found [41.7%, 95% confidence interval (CI): 38.8‒44.7%], followed by Blastocystis sp. (14.1%, 95% CI: 12.1‒16.3%), and Cryptosporidium spp. (1.6%, 95% CI: 0.9‒2.5%). Sequence analyses revealed the presence of assemblages A (7.0%, 3/43) and B (88.4%, 38/43) within G. duodenalis-positive children. Four Cryptosporidium species were detected, including C. hominis (30.8%; 4/13), C. parvum (30.8%, 4/13), C. felis (30.8%, 4/13), and C. viatorum (7.6%, 1/13). Four Blastocystis subtypes were also identified including ST1 (22.7%; 35/154), ST2 (22.7%; 35/154), ST3 (45.5%; 70/154), and ST4 (9.1%; 14/154). Most of the genotyped samples were from asymptomatic children. This is the first report of C. viatorum and Blastocystis ST4 in Mozambique. Molecular data indicate that anthropic and zoonotic transmission (the latter at an unknown rate) are important spread pathways of diarrhoea-causing pathogens in Mozambique.
eng
Blastocystis
Cryptosporidium
Giardia
Mozambique
PCR
Children
Diarrhoea
Enteric parasites
Genotyping
Molecular epidemiology
Molecular Diversity of Giardia duodenalis, Cryptosporidium spp., and Blastocystis sp. in Symptomatic and Asymptomatic Schoolchildren in Zambézia Province (Mozambique).
journal article
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URL
https://repisalud.isciii.es/bitstreams/1eaf77c9-ad50-43fb-a491-0cfe2077db90/download
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application/pdf
MolecularDiversityOfGiardia_2021.pdf
URL
https://repisalud.isciii.es/bitstreams/3afe8b46-9c14-429a-a8be-a91a908df991/download
File
MD5
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text/plain
MolecularDiversityOfGiardia_2021.pdf.txt