2026-07-21T14:00:51Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/125612025-06-25T12:54:53Zcom_20.500.12105_2052com_20.500.12105_2051com_20.500.12105_15322col_20.500.12105_19609col_20.500.12105_16959col_20.500.12105_25179
Repisalud
author
Valdezate, Sylvia
author
Cobo, Fernando
author
Monzon-Fernandez, Sara
author
Medina-Pascual, Maria Jose
author
Zaballos, Ángel
author
Cuesta de la Plaza, Isabel
author
Pino-Rosa, Silvia del
author
Villalón, Pilar
2021-04-08T11:31:43Z
2021-04-08T11:31:43Z
2021-03-16
Antibiotics (Basel). 2021 Mar 16;10(3):304.
10.3390/antibiotics10030304
2079-6382
Antibiotics (Basel, Switzerland)
33809460
http://hdl.handle.net/20.500.12105/12561
Background: Bacteroides fragilis shows high antimicrobial resistance (AMR) rates and possesses numerous AMR mechanisms. Its carbapenem-resistant strains (metallo-β-lactamase cfiApositive) appear as an emergent, evolving clade. Methods: This work examines the genomes, taxonomy, and phylogenetic relationships with respect to other B. fragilis genomes of two B. fragilis strains (CNM20180471 and CNM20200206) resistant to meropenem+EDTA and other antimicrobial agents. Results: Both strains possessed cfiA genes (cfiA14b and the new cfiA28), along with other AMR mechanisms. The presence of other efflux-pump genes, mexAB/mexJK/mexXY-oprM, acrEF/mdtEFtolC, and especially cusR, which reduces the entry of carbapenem via the repression of porin OprD, may be related to meropenem–EDTA resistance. None of the detected insertion sequences were located upstream of cfiA. The genomes of these and other B. fragilis strains that clustered together in phylogenetic analyses did not meet the condition of >95% average nucleotide/amino acid identity, or >70% in silico genome-to-genome hybridization similarity, to be deemed members of the same species, although <1% difference in the genomic G+C content was seen with respect to the reference genome B. fragilis NCTC 9343T. Conclusions: Carbapenem-resistant strains may be considered a distinct clonal entity, and their surveillance is recommended given the ease with which they appear to acquire AMR.
eng
Bacteroides fragilis
Carbapenems
cfiA14
cfiA28
Division II
Porin inactivation
Susceptibility
Genomic Background and Phylogeny of cfiA-Positive Bacteroides fragilis Strains Resistant to Meropenem-EDTA
research article
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GenomicBackgroundAndPhylogeny_2021.pdf
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GenomicBackgroundAndPhylogeny_2021.pdf.txt