2026-07-23T23:05:02Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/125222024-11-29T23:49:54Zcom_20.500.12105_2173com_20.500.12105_2051col_20.500.12105_19597
Repisalud
author
Noguera-Uclés, José Francisco
author
Boyero, Laura
author
Salinas, Ana
author
Cordero Varela, Juan Antonio
author
Benedetti, Johana Cristina
author
Bernabé-Caro, Reyes
author
Sánchez-Gastaldo, Amparo
author
Alonso, Miriam
author
Molina-Pinelo, Sonia
author
Paz Ares, Luis Gonzaga
funder
Regional Government of Andalusia (España)
funder
Instituto de Salud Carlos III
2021-04-06T10:46:07Z
2021-04-06T10:46:07Z
2020-07-27
Cancers (Basel). 2020;12(8):2075
10.3390/cancers12082075
2072-6694
Cancers
32726996
http://hdl.handle.net/20.500.12105/12522
Genomic imprinting is a process that involves one gene copy turned-off in a parent-of-origin-dependent manner. The regulation of imprinted genes is broadly dependent on promoter methylation marks, which are frequently associated with both oncogenes and tumor suppressors. The purpose of this study was to assess the DNA methylation patterns of the imprinted solute-carrier family 22 member 18 (SLC22A18) and SLC22A18 antisense (SLC22A18AS) genes in non-small cell lung cancer (NSCLC) patients to study their relevance to the disease. We found that both genes were hypomethylated in adenocarcinoma and squamous cell carcinoma patients. Due to this imprinting loss, SLC22A18 and SLC22A18AS were found to be overexpressed in NSCLC tissues, which is significantly more evident in lung adenocarcinoma patients. These results were validated through analyses of public databases of NSCLC patients. The reversed gene profile of both genes was achieved in vitro by treatment with ademetionine. We then showed that high SLC22A18 and SLC22A18AS expression levels were significantly associated with worsening disease progression. In addition, low levels of SLC22A18AS were also correlated with better overall survival for lung adenocarcinoma patients. We found that SLC22A18 and SLC22A18AS knockdown inhibits cell proliferation in vitro. All these results suggest that both genes may be useful as diagnostic and prognostic biomarkers in NSCLC, revealing novel therapeutic opportunities.
eng
TUMOR-SUPPRESSOR
SLC22A18
IGF2
EPIGENETICS
TRANSPORTERS
GAIN
The Roles of Imprinted SLC22A18 and SLC22A18AS Gene Overexpression Caused by Promoter CpG Island Hypomethylation as Diagnostic and Prognostic Biomarkers for Non-Small Cell Lung Cancer Patients.
journal article
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TheRolesofImprintedSLC22A18_2020.pdf
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TheRolesofImprintedSLC22A18_2020.pdf.txt