2026-08-10T01:37:38Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/124972024-11-28T17:14:27Zcom_20.500.12105_2173com_20.500.12105_2051com_20.500.12105_15322col_20.500.12105_19597col_20.500.12105_16959

00925njm 22002777a 4500 dc Palacios-Ferrer, José Luis author García-Ortega, María Belén author Gallardo-Gómez, María author García, María Ángel author Díaz, Caridad author Boulaiz, Houria author Valdivia, Javier author Jurado, José Miguel author Almazan-Fernandez, Francisco M author Arias-Santiago, Salvador author Amezcua, Víctor author Peinado, Héctor author Vicente, Francisca author Pérez Del Palacio, José author Marchal, Juan A author Peinado Selgas, Hector author 2021-02-15 Malignant melanoma (MM) is the most aggressive and life-threatening form of skin cancer. It is characterized by an extraordinary metastasis capacity and chemotherapy resistance, mainly due to melanoma cancer stem cells (CSCs). To date, there are no suitable clinical diagnostic, prognostic or predictive biomarkers for this neoplasia. Therefore, there is an urgent need for new MM biomarkers that enable early diagnosis and effective disease monitoring. Exosomes represent a novel source of biomarkers since they can be easily isolated from different body fluids. In this work, a primary patient-derived MM cell line enriched in CSCs was characterized by assessing the expression of specific markers and their stem-like properties. Exosomes derived from CSCs and serums from patients with MM were characterized, and their metabolomic profile was analysed by high-resolution mass spectrometry (HRMS) following an untargeted approach and applying univariate and multivariate statistical analyses. The aim of this study was to search potential biomarkers for the diagnosis of this disease. Our results showed significant metabolomic differences in exosomes derived from MM CSCs compared with those from differentiated tumour cells and also in serum-derived exosomes from patients with MM compared to those from healthy controls. Interestingly, we identified similarities between structural lipids differentially expressed in CSC-derived exosomes and those derived from patients with MM such as the glycerophosphocholine PC 16:0/0:0. To our knowledge, this is the first metabolomic-based study aimed at characterizing exosomes derived from melanoma CSCs and patients' serum in order to identify potential biomarkers for MM diagnosis. We conclude that metabolomic characterization of CSC-derived exosomes sets an open door to the discovery of clinically useful biomarkers in this neoplasia. Mol Oncol. 2021;15(2):407-428. 10.1002/1878-0261.12823 1878-0261 Molecular oncology 33052601 http://hdl.handle.net/20.500.12105/12497 HEPATOCELULAR CARCINOMA THERAPEUTIC TARGET TUMOR BIOMARKERS PLASMA MICROVESICLES REVEALS DISEASE Metabolomic profile of cancer stem cell-derived exosomes from patients with malignant melanoma.