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oai:repisalud.isciii.es:20.500.12105/124782024-09-27T07:58:29Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19605

00925njm 22002777a 4500 dc Nicolás-Ávila, José Ángel author Sanchez-Diaz, Maria author Hidalgo, Andres author 2021-01-26 Cardiac exophers are membrane-bound extracellular vesicles released by cardiomyocytes with varied content and an average diameter of 3.5 μm. Here, we provide a detailed protocol to enable the identification and purification of cardiomyocyte-derived exophers by using fluorescence-activated cell sorting for downstream cellular and molecular analysis. This protocol requires the use of mouse strains expressing fluorescent proteins in cardiomyocytes. STAR Protoc. 2021; 2(1):100286 10.1016/j.xpro.2020.100286 2666-1667 STAR Protocols 33490991 http://hdl.handle.net/20.500.12105/12478 Isolation of exophers from cardiomyocyte-reporter mouse strains by fluorescence-activated cell sorting