2026-06-14T03:54:21Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/122842024-09-27T22:21:17Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609

00925njm 22002777a 4500 dc Formenti, Fabio author Ta Tang, Thuy-Huong author Tamarozzi, Francesca author Silva, Ronaldo author La Marca, Giulia author Pajola, Barbara author Piubelli, Chiara author Perandin, Francesca author Rubio Muñoz, Jose Miguel author Escolar, Eva Marina author Bisoffi, Zeno author Gobbi, Federico author 2021-04 Infections with the filarial nematodes Loa loa and Mansonella perstans are among the most neglected filarial infections. L. loa is endemic in 11 countries of Central and West Africa and loiasis is estimated to affect about 20 million people. M. perstans infection is widespread in more than 30 countries of sub-Saharan Africa. Due to the difficulty in diagnosing loiasis and M. perstans mansonellosis on a clinical basis, the diagnosis of infection with L. loa and M. perstans relies on laboratory techniques. Definitive diagnosis is based on the detection, identification, and quantification of circulating microfilariae (mf) by microscopy of concentrated blood. However, this is impractical for screening purposes as it requires expert laboratory personnel, considerable blood manipulation, and is time consuming, especially for the final issue of negative result reports, which are very common in the population visited outside endemic areas. The aim of the current work is the preliminary evaluation of the performance of the in-house real-time PCR described by Ta and colleagues compared to the routine microscopic approach for the screening of filarial infections in the clinical setting outside endemic areas, using samples from patients accessing the dedicated outpatient clinics for migrants and travelers of a reference centre for tropical diseases in Northern Italy. Acta Trop. 2021 Apr;216:105838. 10.1016/j.actatropica.2021.105838 1873-6254 Acta tropica 33484727 http://hdl.handle.net/20.500.12105/12284 Filarial infections Loa loa Mansonella perstans Molecular diagnosis Real-time PCR ITS1 Microscopy Preliminary comparison between an in-house real-time PCR vs microscopy for the diagnosis of Loa loa and Mansonella perstans