2026-06-14T02:28:38Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/117832025-05-27T10:31:26Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609

00925njm 22002777a 4500 dc Hurtado, A author Alonso, E author Aspiritxaga, I author López Etxaniz, I author Ocabo, B author Barandika, Jesús Félix author Fernández-Ortiz DE Murúa, J I author Urbaneja, F author Álvarez-Alonso, R author Jado, Isabel author García-Pérez, A L author 2017 A Q fever outbreak was declared in February 2016 in a company that manufactures hoists and chains and therefore with no apparent occupational-associated risk. Coxiella burnetii infection was diagnosed by serology in eight of the 29 workers of the company; seven of them had fever or flu-like signs and five had pneumonia, one requiring hospitalisation. A further case of C. burnetii pneumonia was diagnosed in a local resident. Real-time PCR (RTi-PCR) showed a widespread distribution of C. burnetii DNA in dust samples collected from the plant facilities, thus confirming the exposure of workers to the infection inside the factory. Epidemiological investigations identified a goat flock with high C. burnetii seroprevalence and active shedding which was owned and managed by one of the workers of the company as possible source of infection. Genotyping by multispacer sequence typing (MST) and a 10-loci single-nucleotide polymorphism (SNP) discrimination using RTi-PCR identified the same genotype (MST18 and SNP type 8, respectively) in the farm and the factory. These results confirmed the link between the goat farm and the outbreak and allowed the identification of the source of infection. The circumstances and possible vehicles for the bacteria entering the factory are discussed. Epidemiol Infect . 2017 Jul;145(9):1834-1842. 10.1017/S0950268817000796 1469-4409 Epidemiology and infection 28434420 http://hdl.handle.net/20.500.12105/11783 Coxiella burnetii Q fever Dust Genotyping Outbreak Environmental sampling coupled with real-time PCR and genotyping to investigate the source of a Q fever outbreak in a work setting