2026-06-14T03:39:15Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/115952024-11-28T16:22:32Zcom_20.500.12105_15322com_20.500.12105_2051com_20.500.12105_2052col_20.500.12105_16938col_20.500.12105_16982col_20.500.12105_19617
Repisalud
author
Oeo-Santos, Carmen
author
López-Rodríguez, Juan Carlos
author
García-Mouton, Cristina
author
San Segundo-Acosta, Pablo
author
Jurado, Aurora
author
García-Álvarez, Begoña
author
Pérez-Gil, Jesús
author
Villalba, Mayte
author
Barderas Manchado, Rodrigo
author
Cruz, Antonio
author
Moreno-Aguilar, Carmen
funder
Ministerio de Economía y Competitividad (España)
funder
Ministerio de Ciencia, Innovación y Universidades (España)
funder
Comunidad de Madrid (España)
funder
Instituto de Salud Carlos III
funder
Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF)
funder
Regional Government of Andalusia (España)
funder
Fundación Alergosur
2021-01-11T22:36:16Z
2021-01-11T22:36:16Z
2020
Biochim Biophys Acta Biomembr . 2020 Jun 1;1862(6):183258.
10.1016/j.bbamem.2020.183258
Biochimica et biophysica acta. Biomembranes
32142819
http://hdl.handle.net/20.500.12105/11595
Ole e 7 allergen from Olea europaea pollen possesses a major clinical relevance because it produces severe symptoms, such as anaphylaxis, in allergic patients exposed to high olive pollen counts. Ole e 7 is a non-specific lipid transfer protein (nsLTP) characterized by the presence of a tunnel-like hydrophobic cavity, which may be suitable for hosting and, thus, transporting lipids -as it has been described for other nsLTPs-. The identification of the primary amino acid sequence of Ole e 7, and its production as a recombinant allergen, allowed characterizing its lipid-binding properties and its effect at air-liquid interfaces. Fluorescence and interferometry experiments were performed using different phospholipid molecular species and free fatty acids to analyse the lipid-binding ability and specificity of the allergen. Molecular modelling of the allergen was used to determine the potential regions involved in lipid interaction. Changes in Ole e 7 structure after lipid interaction were analysed by circular dichroism. Changes in the IgE binding upon ligand interaction were determined by ELISA. Wilhelmy balance measurements and fluorescence surfactant adsorption tests were performed to analyse the surface activity of the allergen. Using these different approaches, we have demonstrated the ability of Ole e 7 to interact and bind to a wide range of lipids, especially negatively charged phospholipids and oleic acid. We have also identified the protein structural regions and the residues potentially involved in that interaction, suggesting how lipid-protein interactions could define the behaviour of the allergen once inhaled at the airways.
eng
Aeroallergen
nsLTP
Oleic acid
Phospholipids
Interfacial activity
Pulmonary surfactant
Biophysical and biological impact on the structure and IgE-binding of the interaction of the olive pollen allergen Ole e 7 with lipids.
research article
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URL
https://repisalud.isciii.es/bitstreams/2fa7613d-3197-4211-b1e2-7cd8b9330666/download
File
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BiophysicalAndBiologicalImpact_2020.pdf
URL
https://repisalud.isciii.es/bitstreams/e3df4248-307e-4136-9a11-4a85ac22f71d/download
File
MD5
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text/plain
BiophysicalAndBiologicalImpact_2020.pdf.txt