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oai:repisalud.isciii.es:20.500.12105/114062025-06-17T07:29:10Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609

00925njm 22002777a 4500 dc Muñoz-Chimeno, Milagros author Cenalmor, Alejandro author Garcia-Lugo, Maira Alejandra author Hernandez, Marta author Rodríguez-Lázaro, David author Avellón, Ana author 2020-09-15 The hepatitis E virus (HEV) hypervariable region (HVR) presents the highest divergence of the entire HEV genome. It is characteristically rich in proline, and so is also known as the "polyproline region" (PPR). HEV genotype 3 (HEV-3) exhibits different PPR lengths due to insertions, PPR and/or RNA-dependent RNA polymerase (RdRp) duplications and deletions. A total of 723 PPR-HEV sequences were analyzed, of which 137 HEV-3 sequences were obtained from clinical specimens (from acute and chronic infection) by Sanger sequencing. Eight swine stool/liver samples were also analyzed. N- and C-terminal fragments were confirmed as being conserved, but they harbored differences between genotypes and were not proline-plentiful regions. The genuine PPR is the intermediate region between them. HEV-3 PPR contains a higher percentage (30.4%) of prolines than other genotypes. IDRWe describe for the first time: (1) the specific placement of HEV-3 PPR rearrangements in sites 1 to 14 of the PPR, noting that duplications are more frequently attached to sites 11 and 12 (AAs 74-79 and 113-118, respectively); (2) the cadence of repetitions follows a circular-like pattern of blocks A to J, with F, G, H, and I being the most frequent; (3) a previously unreported insertion homologous to apolipoprotein C1; and (4) the increase in frequency of potential N-glycosylation sites and differences in AAs composition related to duplications. Microorganisms . 2020 Sep 15;8(9):1417. 10.3390/microorganisms8091417 2076-2607 Microorganisms 32942608 http://hdl.handle.net/20.500.12105/11406 HEV HVR PPR IDR Hepatitis E virus Hyper-proline Hypervariable Proline-Rich Hypervariable Region of Hepatitis E Virus: Arranging the Disorder