2026-06-14T05:08:07Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/114052025-06-09T11:13:25Zcom_20.500.12105_2052com_20.500.12105_2051com_20.500.12105_15322col_20.500.12105_19609col_20.500.12105_16970col_20.500.12105_16985
Repisalud
author
Gato, Eva
author
Vázquez-Ucha, Juan Carlos
author
Rumbo-Feal, Soraya
author
Álvarez-Fraga, Laura
author
Vallejo, Juan A
author
Martínez-Guitián, Marta
author
Beceiro, Alejandro
author
Ramos Vivas, Jose
author
Sola-Campoy, Pedro Juan
author
Perez-Vazquez, Maria
author
Rodiño-Janeiro, Bruno Kotska
author
Romero, Antonio
author
Poza, Margarita
author
Bou, Germán
author
Oteo-Iglesias, Jesus
author
Perez Gomez, Astrid
funder
Sociedad Española de Enfermedades Infecciosas y Microbiología Clínica
funder
Ministerio de Economía y Competitividad (España)
funder
Instituto de Salud Carlos III
funder
RETICS-Investigación en Patología Infecciosa (REIPI-ISCIII) (España)
funder
Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF)
2020-11-24T10:16:17Z
2020-11-24T10:16:17Z
2020
Proc Natl Acad Sci U S A. 2020 Jul 21;117(29):17249-17259.
10.1073/pnas.1921393117
1091-6490
Proceedings of the National Academy of Sciences of the United States of America
32641516
http://hdl.handle.net/20.500.12105/11405
Control of infections caused by carbapenem-resistant Klebsiella pneumoniae continues to be challenging. The success of this pathogen is favored by its ability to acquire antimicrobial resistance and to spread and persist in both the environment and in humans. The emergence of clinically important clones, such as sequence types 11, 15, 101, and 258, has been reported worldwide. However, the mechanisms promoting the dissemination of such high-risk clones are unknown. Unraveling the factors that play a role in the pathobiology and epidemicity of K. pneumoniae is therefore important for managing infections. To address this issue, we studied a carbapenem-resistant ST-15 K. pneumoniae isolate (Kp3380) that displayed a remarkable adherent phenotype with abundant pilus-like structures. Genome sequencing enabled us to identify a chaperone-usher pili system (Kpi) in Kp3380. Analysis of a large K. pneumoniae population from 32 European countries showed that the Kpi system is associated with the ST-15 clone. Phylogenetic analysis of the operon revealed that Kpi belongs to the little-characterized γ2-fimbrial clade. We demonstrate that Kpi contributes positively to the ability of K. pneumoniae to form biofilms and adhere to different host tissues. Moreover, the in vivo intestinal colonizing capacity of the Kpi-defective mutant was significantly reduced, as was its ability to infect Galleria mellonella The findings provide information about the pathobiology and epidemicity of Kpi+K. pneumoniae and indicate that the presence of Kpi may explain the success of the ST-15 clone. Disrupting bacterial adherence to the intestinal surface could potentially target gastrointestinal colonization.
eng
GI tract colonization
Klebsiella pneumoniae
ST-15 high-risk clone
Chaperone-usher pili system
Pathogenesis
Kpi, a chaperone-usher pili system associated with the worldwide-disseminated high-risk clone Klebsiella pneumoniae ST-15
research article
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KpiAChaperoneUsher_2020.pdf
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https://repisalud.isciii.es/bitstreams/241152bd-9843-422b-a694-bf1e6d5acac3/download
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text/plain
KpiAChaperoneUsher_2020.pdf.txt