2026-06-16T14:02:52Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/114032024-11-30T00:41:34Zcom_20.500.12105_2173com_20.500.12105_2051col_20.500.12105_19597
Repisalud
author
Mortuza, Gulnahar B
author
Cavazza, Tommaso
author
Garcia-Mayoral, Maria Flor
author
Hermida, Dario
author
Peset, Isabel
author
Pedrero, Juan G
author
Merino, Nekane
author
Blanco, Francisco J
author
Lyngsø, Jeppe
author
Bruix, Marta
author
Pedersen, Jan Skov
author
Vernos, Isabelle
author
Montoya, Guillermo
funder
Comunidad de Madrid (España)
funder
Fundación Ramón Areces
funder
Ministerio de Economía y Competitividad (España)
funder
ICREA
funder
Danish Research Council
2020-11-24T10:10:01Z
2020-11-24T10:10:01Z
2014-09-29
Nat Commun . 2014;5:5072.
10.1038/ncomms6072
2041-1723
Nature communications
25262927
http://hdl.handle.net/20.500.12105/11403
chTOG is a conserved microtubule polymerase that catalyses the addition of tubulin dimers to promote microtubule growth. chTOG interacts with TACC3, a member of the transforming acidic coiled-coil (TACC) family. Here we analyse their association using the Xenopus homologues, XTACC3 (TACC3) and XMAP215 (chTOG), dissecting the mechanism by which their interaction promotes microtubule elongation during spindle assembly. Using SAXS, we show that the TACC domain (TD) is an elongated structure that mediates the interaction with the C terminus of XMAP215. Our data suggest that one TD and two XMAP215 molecules associate to form a four-helix coiled-coil complex. A hybrid methods approach was used to define the precise regions of the TACC heptad repeat and the XMAP215 C terminus required for assembly and functioning of the complex. We show that XTACC3 can induce the recruitment of larger amounts of XMAP215 by increasing its local concentration, thereby promoting efficient microtubule elongation during mitosis.
eng
XTACC3-XMAP215 association reveals an asymmetric interaction promoting microtubule elongation.
journal article
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XTACC3–XMAP215association_2014.pdf
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XTACC3–XMAP215association_MOESM1337_ESM.pdf
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XTACC3–XMAP215association_2014.pdf.txt
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XTACC3–XMAP215association_MOESM1337_ESM.pdf.txt