2026-06-14T03:28:57Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/113552024-09-27T21:13:25Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609
Repisalud
author
González-Serrano, Lydia
author
Muñoz-Algarra, María
author
Gonzalez-Sanz, Ruben
author
Portero-Azorín, María Francisca
author
Amaro, María José
author
Higueras, Purificación
author
Cabrerizo, Maria
2020-11-17T13:01:44Z
2020-11-17T13:01:44Z
2020
J Clin Virol . 2020 Jul;128:104420
10.1016/j.jcv.2020.104420
1873-5967
Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology
32454428
http://hdl.handle.net/20.500.12105/11355
. Viral infections are recognized as the most common cause of acute gastroenteritis (AGE). Virus detection by immune analytical methods is recommended for diagnosis because of its simplicity and low cost.
. Two commercial immunochromatographic (ICG) techniques (Materlab) for rapid detection of rotavirus/adenovirus and norovirus respectively, were evaluated by comparison to the results obtained using PCR methods. In addition, clinical and epidemiologic characteristics of AGE infections have been described.
. A total of 100 faecal samples collected from patients with AGE (84% children) admitted into a Spanish Hospital between February and July 2018, were studied for rotavirus-A, adenovirus and norovirus GI/GII by the ICG tests as well as by PCR and sequencing. Other enteric viruses (enterovirus and astrovirus) were investigated by PCR methods. Gastrointestinal bacteria and parasites were also tested.
. Evaluated ICG tests yielded high specificity (>97%). Sensitivity values were high for rotavirus/adenovirus (>80%) but lower for norovirus (57%). Overall, and taking into account coinfections, viruses (32%), bacteria (14%) and parasites (1%) could be detected. Rotavirus-A were the most frequently identified viruses (16%), followed by enterovirus (12%), norovirus (4%), adenovirus 41 (4%) and astrovirus (1%). In five vaccinated children, a rotavirus was detected.
. ICG technique is a useful tool for the routine diagnosis of AGE infections at hospital, but for surveillance and epidemiological studies, it is needed the use of amplification and sequencing methods, which also allow monitoring of new strains or variants emergence. In this study, an etiological pathogen was determined only in 44% of samples.
eng
Adenovirus
Immunochromatographic test
Norovirus
PCR
Rotavirus
Viral gastroenteritis in hospitalized patients: Evaluation of immunochromatographic methods for rapid detection in stool samples.
research article
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ViralGastroenteritisInHospitalized_2020.pdf.txt