2026-06-14T03:42:51Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/113462024-11-29T21:33:58Zcom_20.500.12105_2173com_20.500.12105_2051col_20.500.12105_19597
Repisalud
author
Carreras, Pilar
author
Gonzalez, Iciar
author
Ortiz-Ruiz, Alejandra
author
Gallardo, Miguel
author
Martinez-Lopez, Joaquin
funder
Unión Europea. Comisión Europea. H2020
funder
Marie Curie
2020-11-16T09:24:00Z
2020-11-16T09:24:00Z
2020-02-29
Micromachines (Basel). 2020 ;11(3):261.
10.3390/mi11030261
Micromachines
32121351
http://hdl.handle.net/20.500.12105/11346
We previously reported a new approach for micromanipulation and encapsulation of human stem cells using a droplet-based microfluidic device We demonstrated the possibility of encapsulating and culturing difficult-to-preserve primary human hematopoietic stem cells using an engineered double layered bead composed by an inner layer of alginate and an outer layer of puramatrix constructed using a soft technology without the use of any external force. In this work, we use this micro manipulation technique to build a 3D scaffold as a biomimetic model to recapitulate the niche of patient-derived multiple myeloma cells (MM cell) using a multilayered 3D tissue scaffold constructed in a microfluidic device and cultured in 10% FBS culture medium. In the current study, we included the use of this biomimetic model comprising supporting human Mesenchymal stem cells to show the mid-term survival of MM cells in the proposed structures. We found that the generated microniches were suitable for the maintenance of MM cells with and without supporting cells. Additionally, cultured MM cells in droplets were exposed to both Bortezomib and Lenalidomide to test their toxicity in the cultured patient derived cells. Results indicate that the maintained MM cells were consistently responding to the applied medication, opening a wide field of possibilities to use the presented micro device as an ex vivo platform for drug screening.
eng
DIFFERENTIATION
CULTURE
MODEL
INTERLEUKIN-6
HYDROGELS
Droplet Microfluidics for the ex Vivo Expansion of Human Primary Multiple Myeloma Cells.
journal article
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URL
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File
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application/pdf
DropletMicrofluidicsfortheexVivo_2020.pdf
URL
https://repisalud.isciii.es/bitstreams/33747d76-0909-4f58-b948-e8bca5175978/download
File
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text/plain
DropletMicrofluidicsfortheexVivo_2020.pdf.txt