2026-06-14T03:51:06Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/113362024-09-27T21:57:06Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609col_20.500.12105_19619
Repisalud
author
Medina-Pascual, Maria Jose
author
Monzon-Fernandez, Sara
author
Villalón, Pilar
author
Cuesta de la Plaza, Isabel
author
González-Romo, Fernando
author
Valdezate, Sylvia
2020-11-13T13:04:31Z
2020-11-13T13:04:31Z
2020-03
Int J Syst Evol Microbiol . 2020 Mar;70(3):2016-2025.
10.1099/ijsem.0.004010
1466-5034
International journal of systematic and evolutionary microbiology
32003711
http://hdl.handle.net/20.500.12105/11336
The taxonomic position of an unknown bacterial strain designated CNM695-12, isolated from the blood of an immunocompromised subject, was investigated via phenotypic, chemotaxonomic, genotypic and genomic analyses. Bacterial cells were determined to be Gram-stain-negative bacilli, aerobic, non-motile and non-spore-forming. The strain showed catalase activity but no oxidase activity. Optimal growth occurred at 37 °C, pH 7 and with 0-1 % NaCl. C16 : 0, summed feature 8 (comprising C18 : 1ω7c /C18:1 ω6c), and C18 : 1ω9c were the most abundant fatty acids, and ubiquinone 8 was the major respiratory quinone. The polar lipids present included phosphatidylglycerol, phosphatidylethanolamine and other aminophospholipids. The 16S rRNA gene sequence showed approximately 93.5 % similarity to those of different species with validly published names within the order Burkholderiales (e.g. Leptothrix mobilis Feox-1T, Aquabacterium commune B8T, Aquabacterium citratiphilum B4T and Schlegelella thermodepolymerans K14T). Phylogenetic analyses based on 16S rRNA gene sequences and concatenated alignments including the sequences for 107 essential proteins, revealed the strain to form a novel lineage close to members of the family Comamonadaceae. The highest average nucleotide identity and average amino acid identity values were obtained with Schlegelella thermodepolymerans K14T (69.6 and 55.7 % respectively). The genome, with a size of 3.35 Mb, had a DNA G+C content of 52.4 mol% and encoded 3056 predicted genes, 3 rRNA, 1 transfer-messengerRNA and 51 tRNA. Strain CNM695-12 thus represents a novel species belonging to a novel genus within the order Burkholderiales, for which the name Saezia sanguinis gen. nov., sp. nov. is proposed. The type strain is CNM695-12T (=DSM 104959T=CECT 9208T).
eng
Betaproteobacteria
Burkholderiales
Comamonadaceae
Saezia
Sanguinis
Saezia sanguinis gen. nov., sp. nov., a Betaproteobacteria member of order Burkholderiales, isolated from human blood.
research article
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URL
https://repisalud.isciii.es/bitstreams/62513fe7-bd46-4ddf-9546-cb666d30a6ca/download
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SaeziaSanguinisGenNov_2020.pdf
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https://repisalud.isciii.es/bitstreams/502e4237-2b06-4d2a-b05d-c3a1b24827ca/download
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510f42cebe6a61d37e34a5d187902bd6
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text/plain
SaeziaSanguinisGenNov_2020.pdf.txt